食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 272-276.doi: 10.7506/spkx1002-6300-200920058

• 分析检测 • 上一篇    下一篇

双孢蘑菇RAPD标记的遗传多样性分析

林 媛1,陈文炳2,*,邵碧英2,王泽生3,廖剑华3,李寿崧2,江树勋2   

  1. 1.福建农林大学金山学院 2.福建出入境检验检疫局 3.福建省农业科学院
  • 收稿日期:2009-07-07 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 陈文炳2,*, E-mail:621213wbc@163.com
  • 基金资助:

    国家质量监督检验检疫总局科技项目(2007IK159)

Detection of Genetic Diversity of Agaricus bisporus Using RAPD Markers

LIN Yuan1,CHEN Wen-bing2,*,SHAO Bi-ying2,WANG Ze-sheng3,LIAO Jian-hua3,LI Shou-song2,JIANG Shu-xun2   

  1. 1. Jinshan College, Fujian Agriculture and Forestry University, Fuzhou 350002, China ;2. Fujian Entry-Exit Inspection and
    Quarantine Bureau, Fuzhou 350001, China ;3. Fujian Academy of Agricultural Sciences, Fuzhou 350014, China
  • Received:2009-07-07 Online:2009-10-15 Published:2010-12-29
  • Contact: CHEN Wen-bing2,*, E-mail:621213wbc@163.com

摘要:

随机抽取108个随机引物对40个双孢蘑菇栽培菌株的DNA进行RAPD-PCR扩增实验,发现有71个随机引物能够扩增出清晰的、稳定的、具有多态性的RAPD条带。结果表明,71个随机引物共扩增出567条DNA带,平均每个引物扩增出7.99条DNA带,其中434条DNA带具有多态性,占总数的76.5%。基于RAPD-PCR扩增资料,采用欧氏距离平方系数和组间连接法,应用SPSS 11.0软件进行聚类分析,探讨40个品种间的亲缘关系与类群划分。当取欧氏距离平方系数阈值为20.5时,所有品种分为三大类;当取欧氏距离平方系数阈值取8.5时,40种双孢蘑菇菌株可被分为6个类群,此结果与双孢蘑菇菌株群的同工酶分类研究结果相当吻合。

关键词: 蘑菇, 随机扩增多态性DNA(RAPD), 遗传多样性, 聚类分析, 亲缘关系

Abstract:

The genetic diversity of 40 strains of Agaricus bisporus was assessed using RAPD markers. Of 108 random primers, 71 were informative and yielded 567 bands, 434 of which were polymorphic with a mean of 7.99 bands per primer. Cluster analysis was performed by between-groups linkage method and coefficient of euclidean distance square (CEDS) method using SPSS 11.0. As a result, these Agaricus bisporus strains could be clustered into 3 groups at 20.5 in CEDS, or could be clustered into 6 groups at 8.5 in CEDS, which is in considerable agreement with the results obtained by isoenzyme clustering.

Key words: Agaricus bisporus, random amplified polymorphic DNA (RAPD), genetic diversity, cluster analysis, genetic relationship

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