关键词: 高压, 啤酒酵母, 随机扩增多态性DNA(RAPD)

Abstract:

High pressure-induced mutation of beer yeast (Saccharomyces cerevias) was studied. The optimal values of mutagenesis parameters were determined. Meanwhile, the original and mutant strains of bear yeast were compared for differences in fermentation performance and RAPD fingerprint. Results indicated that a mutant strain, MS-3, was obtained under the optimal technological conditions of treatment with 300 MPa for 15 min. Totally 7 arbitrary primers and 4 arbitrary primers could result in relatively high DNA amplification and obvious mutant characteristics. Therefore, these investigations are helpful to provide a basis for screening and breeding of excellent bear yeast strains by mean of high-pressure mutagenesis.

Key words: high pressure, Saccharomyces cerevias, random amplified polymorphic DNA (RAPD)