食品科学 ›› 2010, Vol. 31 ›› Issue (4): 35-38.doi: 10.7506/spkx1002-6300-201004008

• 工艺技术 • 上一篇    下一篇

枫香树叶总黄酮提取工艺优化及含量动态变化

刘亚敏1,刘玉民1,马 明1,李昌晓2,练彩霞1,曹淑君1   

  1. 1.西南大学资源环境学院 2.西南大学生命科学学院
  • 收稿日期:2009-04-10 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 刘亚敏 E-mail:yaminliu0511@163.com
  • 基金资助:

    重庆市自然科学基金重点资助项目(CSTC-2008BA7032);“十五” 国家科技攻关项目(2004BA604A05)

Extraction and Dynamic Content of Total Flavonoids in Leaves of Liquidambar formosana Hance

LIU Ya-min1,LIU Yu-min1,MA Ming1,LI Chang-xiao2,LIAN Cai-xia1,CAO Shu-jun1   

  1. 1. College of Resources and Environment, Southwest University, Chongqing 400715, China;
    2. College of Life Sciences, Southwest University, Chongqing 400715, China
  • Received:2009-04-10 Online:2010-02-15 Published:2010-12-29
  • Contact: LIU Ya-min E-mail:yaminliu0511@163.com

摘要:

以枫香叶为原料,分析乙醇体积分数、提取温度、提取时间、固液比等因素对枫香叶总黄酮含量的影响,以L9(34)正交试验方法优化总黄酮提取工艺,然后在该工艺条件下考察不同采叶时期枫香叶总黄酮含量的动态变化。结果表明:乙醇体积分数、提取温度、提取时间、固液比对枫香叶总黄酮含量均有影响;枫香叶中总黄酮提取的最佳工艺组合为用体积分数60% 的乙醇以1:30(g/mL)的固液比在80℃条件下提取2.5h;采叶时间对枫香叶黄酮含量影响较大,4 月份为最佳采摘时间。

关键词: 枫香叶, 总黄酮, 工艺优化, 含量变化

Abstract:

Leaves of Liquidambar formosana Hance were used as the raw material to extract total flavonoids and the dynamic change of total flavonoids in leaves of Liquidambar formosana Hance during growth was examined. To maximize the extraction of total flavonoids, technological parameters like ethanol concentration, temperature, extraction time and solid/liquid ratio were investigated by single factor method coupled with L9(34) orthogonal array design. Results showed that the maximum extraction of total flavonoids was obtained by using 60% ethanol to extract leaves of Liquidambar formosana Hance for 2.5 h at 80 ℃ with a solid/liquid ratio of 1:30. Total flavonoids exhibited an obvious change as leaves of Liquidambar formosana Hance grew, and a maximum content was observed in April.

Key words: leaves of Liquidambar formosana Hance, total flavonoids, optimization, kinetics

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