食品科学 ›› 2010, Vol. 31 ›› Issue (4): 39-41.doi: 10.7506/spkx1002-6300-201004009

• 工艺技术 • 上一篇    下一篇

正交试验法优化黑米黑色素的超声辅助提取工艺

张吉祥1,赵文静1,白晓杰1,欧来良2 ,*   

  1. 1.廊坊师范学院化学与材料科学学院 2.南开大学分子生物学研究所,生物活性材料教育部重点实验室
  • 收稿日期:2009-04-27 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 欧来良 E-mail:zhangjixiang1973@yahoo.com.cn
  • 基金资助:

    河北省教育厅基金资助项目(2007317);廊坊师范学院科学基金资助项目(LSZB200801)

Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment

ZHANG Ji-xiang1,ZHAO Wen-jing1,BAI Xia-jie1,OU Lai-liang2,*   

  1. 1. Faculty of Chemistry and Material Science, Langfang Teachers College, Langfang 065000, China;2. Key Laboratory of
    Bioactive Materials, Ministry of Education, Institute of Molecular Biology, Nankai University, Tianjin 300071, China
  • Received:2009-04-27 Online:2010-02-15 Published:2010-12-29
  • Contact: OU Lai-liang E-mail:zhangjixiang1973@yahoo.com.cn

摘要:

以黑米为原料,优化黑色素的最佳超声辅助提取工艺。采用正交试验法,研究超声波功率、浸提时间、浸提剂体积分数及料液比(黑米︰乙醇溶液,m/V)对黑米色素提取率的影响。结果表明:在所考察的因素中,黑米黑色素的最佳提取工艺为A3B1C3D3。浸提黑色素宜选体积分数80% 的乙醇溶液作为提取剂,超声波作用时间50min,料液比1:32,超声功率250W,该优化方法的提取率为4.5%,比传统的索式提取法的提取率提高了近3 倍。

关键词: 黑米, 黑色素, 正交试验法, 超声提取

Abstract:

Ethanol was used for extracting black rice pigment under ultrasound assistance. To maximize the extraction of black rice pigment, orthogonal array design was employed for investigating the effects of ethanol concentration, solid-to-liquid ratio, ultrasound power, extraction time on black rice pigment yield (represented by the absorbance at 543 nm wavelength of extract solution). Results showed that the use of 80% ethanol for extracting black rice grains at 1:32 solid-to-liquid ratio (m/V) with the assistance of 50 min ultrasound treatment at 250 W gave an optimal pigment extraction and the resulting pigment yield reached up to 4.5%, with a near 3-fold increase in comparison with the conventional Soxhlet extraction.

Key words: black rice, pigment, orthogonal array design, ultrasound-assisted extraction

中图分类号: