食品科学 ›› 2010, Vol. 31 ›› Issue (4): 183-186.doi: 10.7506/spkx1002-6300-201004041

• 分析检测 • 上一篇    下一篇

冬瓜中DEHP 气相色谱- 质谱联用检测方法的建立

王家文,杜琪珍* ,夏会龙,傅小伟   

  1. 浙江工商大学食品与生物工程学院
  • 收稿日期:2009-04-21 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 杜琪珍 E-mail:qizhendu@126.com
  • 基金资助:

    浙江省自然科学基金杰出青年人才项目(R507719);高等学校博士学科点专项科研基金项目(20060353004);
    国家自然科学基金项目(20977084)

GC-MS Determination of Di-2-ethylhexyl Phthalate in Fruits of Benincasa hispida

WANG Jia-wen,DU Qi-zhen*,XIA Hui-long,FU Xiao-wei   

  1. College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China
  • Received:2009-04-21 Online:2010-02-15 Published:2010-12-29
  • Contact: Qizhen Du E-mail:qizhendu@126.com

摘要:

对冬瓜中邻苯二甲酸二(2- 乙基己基)酯(DEHP)的含量测定进行较为系统的研究,建立气相色谱- 质谱联用测定冬瓜中DEHP 含量的方法。结果表明:方法检出限(RSN = 3)为2.65 × 10-3mg/kg,线性范围为0.0001~1.0mg/mL,加标回收率为87.69%~96.85%,平均相对标准偏差(RSD)为3.16%。该分析方法具有准确度高、选择性好、干扰少等特点,能满足冬瓜中DEHP 的分析要求,具有实际的应用检测价值。

关键词: 邻苯二甲酸二(2- 乙基己基)酯, 气相色谱- 质谱联用, 冬瓜, 检测

Abstract:

Benincasa hispida exhibits strong accumulation capacity towards di-2-ethylhexyl phthalate (DEPH), which proves to be carcinogenic. In the present study, a gas chromatography coupled with mass spectrometric method (GC-MS) was proposed for the determination of DEPH in fruits of Benincasa hispida, based on optimal DEPH extraction process. The developed standard curve of DEPH exhibited a good linearity over the range of 0.0001 to 1.0 mg/mL. The limit of detection (RS/N = 3) was 2.65×10-3 mg/kg. The recoveries for DEPH at 5 spike levels were between 87.69% and 96.85%, with an average RSD of 3.16%. The proposed method, thanks to its high accuracy, good selectivity and less interference, meets the requirements for the analysis of DEHP in fruits of Benincasa hispida so as to have a great application prospect.

Key words: DEHP, gas chromatography-mass spectrometry (GC-MS), Benincasa hispida, determination

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