食品科学 ›› 2010, Vol. 31 ›› Issue (4): 194-197.doi: 10.7506/spkx1002-6300-201004044

• 分析检测 • 上一篇    下一篇

辣椒油中苯并芘参考标准物质的制备与定值

郭 翠1,王建华2 , 1 ,* ,杜恒清2,陈世山2,昃向君2,隋玉柱1   

  1. 1.青岛大学化学化工与环境学院 2.山东出入境检验检疫局
  • 收稿日期:2009-03-31 修回日期:2009-08-13 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 王建华 E-mail:whywrs@yahoo.com.cn
  • 基金资助:

    山东出入境检验检疫局科研项目(SK200856)

Preparation, Testing and Certification of Reference Materials for Benzo(a)pyrene in Pepper Oil

GUO Cui1,WANG Jian-hua2,1,*,DU Heng-qing2,CHEN Shi-shan2,ZE Xiang-jun2,SUI Yu-zhu1   

  1. 1. Institute of Chemical Engineering and Environment, Qingdao University, Qingdao 266071, China;
    2. Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China
  • Received:2009-03-31 Revised:2009-08-13 Online:2010-02-15 Published:2010-12-29
  • Contact: WANG Jian-hua E-mail:whywrs@yahoo.com.cn

摘要:

为建立辣椒油中苯并芘标准物质的研制和定值方法,向辣椒油中添加一定量的苯并芘标准溶液,搅拌均匀后分装100 瓶,随机抽取10 瓶样品进行样品均匀性检验,在95% 置信区间内,检验结果同时通过F 检验和t 检验。在恒温4℃冷藏和常温20℃条件下,在8 周内进行样品的稳定性检验,检验结果通过t 检验,在95% 置信区间内。并通过分析标准物质制备过程中的不确定度来源,计算出不确定度分量、合成标准不确定度及扩展不确定度。制备的辣椒油中苯并芘采用同位素稀释气相色谱- 质谱联用法进行辣椒油中苯并芘定值,定值后的辣椒油样品中苯并芘含量为(9.98 ± 0.914)μg/kg(k = 2)。本标准物质对于植物油中苯并芘检测的方法验证和质量控制具有重要意义。

关键词: 食品安全, 标准物质, 苯并芘, 辣椒油, 质量保证

Abstract:

A methodology regarding preparation and certification of the reference material for Benzo(a)pyrene in pepper oil was presented. Spiked pepper oil was packed in 100 bottles after homogenization and 10 of these 100 bottles were selected to test the homogeneity of the sample. Two batches of 10 samples from these 100 bottles were kept at 4 ℃ and 20 ℃, respectively. The stabilities of 20 bottles were analyzed e once a week for 8 consecutive weeks. Test results demonstrate the homogeneity and stability of that the pepper oil sample. Benzo(a)pyrene was determined by gas chromatography-mass spectrometry, based on isotope dilution. Uncertainty components, combined standard uncertainty and expanded uncertainty were also calculated, and the concentration of the sample was certified as (9.98 ± 0.914 )μg/kg, k = 2. The reference material is intended for use in the methodological validation and quality control for benzo(a)pyrene in edible vegetable oils

Key words: food safety, reference material, benzo(a)pyrene(Bap), pepper oil, quality assurance

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