食品科学 ›› 2010, Vol. 31 ›› Issue (4 ): 230-232.doi: 10.7506/spkx1002-6300-201004053

• 分析检测 • 上一篇    下一篇

高效液相色谱- 串联质谱法测定猪肉中10 种类固醇类激素残留

牛晋阳,孙 焕,李莹莹   

  1. 中国肉类食品综合研究中心
  • 收稿日期:2009-10-30 出版日期:2010-02-15 发布日期:2010-12-29

High-performance Liquid Chromatography Coupled with Tandem Mass Spectrometric Determination of Steroid Hormone Multi-Residues in Pork

NIU Jin-yang,SUN Huan,LI Ying-ying   

  1. China Meat Research Center, Beijing 100068, China
  • Received:2009-10-30 Online:2010-02-15 Published:2010-12-29

摘要:

建立猪肉中类固醇类激素残留高效液相色谱- 串联质谱(LC-MS/MS)分析方法。样品经甲醇提取,固相萃取柱净化后采用LC-MS/MS 进行检测分析;并对样品前处理条件和质谱参数进行研究。结果表明:这些激素物质的线性范围均为0.5~100μg/kg;低、中、高3 种加标水平的加标回收率为71%~89%;最低检出限为0.2~2μg/kg。本方法快速简便、灵敏度高、选择性好、测定结果令人满意,可在各食品检测机构推广应用。

关键词: 类固醇类激素, 猪肉, 高效液相色谱- 串联质谱

Abstract:

A method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed for the determination of steroid hormone multi-residues in pork. Analytes were extracted from samples with methanol and cleaned up using a solid-phase extraction cartridge prior to HPLC-MS/MS analysis. The proposed method exhibited a good linearity for 10 steroid hormones over the range of 0.5-100μg/kg and a limit of detection ranging from 0.2 to 2μg/kg. Mean recoveries for 10 steroid hormones in blank pork samples spiked at low, medium and high concentrations were between 71% and 88%, with relative standard deviations below 10%. The method is characteristic of rapidity, simplicity, good selectivity and high sensitivity, and therefore has a great popularization potential.

Key words: steroid hormone, pork, HPLC/MS-MS

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