食品科学 ›› 2010, Vol. 31 ›› Issue (7): 251-255.doi: 10.7506/spkx1002-6300-201007055

• 营养卫生 • 上一篇    下一篇

无斑鹞鲼肉对大鼠脂质代谢的影响

王玉明,胡晓倩,任兵兴,王静凤,薛长湖*   

  1. 中国海洋大学食品科学与工程学院
  • 收稿日期:2009-08-27 出版日期:2010-04-01 发布日期:2010-12-29
  • 通讯作者: 薛长湖 E-mail:xuech@ouc.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2008BAD94B05);国家自然科学基金项目(30972285)

Effect of Dietary Aetobatus flagelum on Lipid Metabolism in Rats

WANG Yu-ming,HU Xiao-qian,REN Bing-xing,WANG Jing-feng,XUE Chang-hu*   

  1. (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
  • Received:2009-08-27 Online:2010-04-01 Published:2010-12-29
  • Contact: XUE Chang-hu E-mail:xuech@ouc.edu.cn

摘要:

目的:研究无斑鹞鲼肉对大鼠脂质代谢的影响,以提高低值水产品的利用价值。方法:将SD 大鼠分3 组,对照组(酪蛋白)、10% 鹞鲼肉组(酪蛋白+ 鹞鲼肉)和20% 鹞鲼肉组。分别测定大鼠血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、游离脂肪酸(FFA)浓度,以及肝脏TG、TC、磷脂(PL)浓度,并测定肝脏脂质代谢相关酶——葡萄糖-6- 磷酸(G6PDH)脱氢酶、苹果酸酶、脂肪酸合成酶、磷脂酸磷酸酶和肉碱棕榈酰转移酶的活性。结果:摄食鹞鲼肉可显著降低大鼠血清TG、TC 和FFA 含量(P < 0.05),对血清HDL-C 浓度无明显影响,但能显著降低大鼠动脉硬化指数(AI)。另外,鹞鲼肉可明显降低肝脏TG 和TC 含量(P < 0.05),抑制肝脏脂肪合成相关酶的活性,但对脂肪酸分解酶活性则未见明显影响,同时FAS、CPT-1、CPT-2 mRNA 表达量也无明显变化。结论:摄食无斑鹞鲼肉具有降低大鼠血清和肝脏脂肪含量的作用,主要是通过抑制脂肪酸和TG 的合成实现。

关键词: 鹞鲼, 鱼肉蛋白, 降血脂, 脂质代谢, 酶活性

Abstract:

Objective: To investigate the effect of dietary Aetobatus flagelum on lipid metabolism in rats for improving the utilization value of low-value aquatic products. Methods: Twenty-one male SD rats were randomly divided into 3 groups, including normal control group (fed normal diet containing casein), low-dose treatment group (fed normal diet containing 10% of Aetobatus flagelum meat powder and 10% of casein) and high-dose treatment group (fed normal diet containing 20% of Aetobatus flagelum). After 28 days of consecutive administration, all mice were sacrificed to measure triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), free fatty acid (FFA) contents in serum and TG, TC and phospholipids contents in liver and the activities of enzymes related to lipids metabolism, such as malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH), fatty acid synthase (FAS), phosphatidic acid phosphophydrolase (PAP) and carnitin palmitoyl transferase (CPT). Results: Compared with the control group, serum TG, TC and FFA levels exhibited a significant decrease in both the treatment groups (P < 0.05). Both low- and high-dose treatments had no obvious effect on serum HDL-C level but resulted in an obvious reduction of arteriosclerosis index in rats. Moreover, a significant decrease in TG and TC contents in liver was observed in both the treatment groups when compared with the control group (P < 0.05). The activities of G6PDH, ME, FAS and PAP in liver were all inhibited by dietary Aetobatus flagelum, whereas no obvious effects on the activity of hepatic CPT and mRNA expression of FAS, CPT-1, CPT-2 genes were observed. Conclusion: The above results indicate that the reduction of serum lipids by dietary Aetobatus flagelum is related to the down-regulation of hepatic lipids synthesis. Dietary Aetobatus flagelum can attenuate the accumulation of serum and hepatic lipids through reducing the synthesis of hepatic fatty acid and TG.

Key words: Aetobatus flagellum, hypolipidemic effect, lipid metabolism, amino acid composition, enzyme activity

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