食品科学 ›› 2009, Vol. 30 ›› Issue (10 ): 239-241.doi: 10.7506/spkx1002-6630-200910056

• 分析检测 • 上一篇    下一篇

大兴安岭笃斯越橘花色苷成分鉴定研究

胡济美,籍保平* ,周 峰,韩 勇,水明磊   

  1. 中国农业大学食品科学与营养工程学院
  • 收稿日期:2009-03-03 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 籍保平* E-mail:jbp@cau.edu.cn

Identification of Anthocyanins from Daxing’anling Wild Vaccinium uliginosum Fruit

HU Ji-mei,JI Bao-ping*,ZHOU Feng,HAN Yong,SHUI Ming-lei   

  1. (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Received:2009-03-03 Online:2009-05-15 Published:2010-12-29
  • Contact: JI Bao-ping*, E-mail:jbp@cau.edu.cn

摘要:

对东北野生笃斯越橘中花色苷进行提取,并采用pH 示差法进行定性和定量分析。再对粗提液中的花色苷进行分离纯化,通过HPLC-MS 对其花色苷种类和结构进行鉴定,并最终鉴定出13 种花色苷。

关键词: 笃斯越橘, 花色苷, 分离, 鉴定

Abstract:

In this study, anthocyanins were extracted from Daxing’anling wild Vaccinium uliginosum fruit with 70% ethanol under the assistance of unltrasonic, and were subjected to qualitative and quantitative analysis by pH differential method. Furthermore, the extracts were separated into phenolic acids, anthocyanins and flavanones on Sephadex LH-20 column with different elution solutions. 13 kinds of anthocyanins were identified by HPLC-MS.

Key words: Vaccinium uliginosum, anthocyanins, extraction, identification

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