食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 292-295.doi: 10.7506/spkx1002-6630-200918066

• 分析检测 • 上一篇    下一篇

福林- 酚比色法测定桑椹中总多酚

李巨秀,王柏玉   

  1. 西北农林科技大学食品科学与工程学院
  • 收稿日期:2009-06-08 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 李巨秀 E-mail:juxiuli@msn.com
  • 基金资助:

    西北农林科技大学校长基金资助项目

Folin-Ciocalteu Colorimetric Determination of Total Polyphenols in Mulberry Fruits

LI Ju-xiu,WANG Bai-yu   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Received:2009-06-08 Online:2009-09-15 Published:2010-12-29
  • Contact: LI Ju-xiu E-mail:juxiuli@msn.com

摘要:

以桑椹为原料,没食子酸为标准物质研究了用福林- 酚比色法测定桑椹提取液中多酚类物质含量的最适反应条件。结果表明,当福林- 酚试剂与质量分数为12% 的Na2CO3 溶液体积比为1:2、反应温度20℃、反应时间2h 时,总多酚的含量与吸光度呈良好的线性关系,测定方法的平均回收率为97.63%,相对标准偏差(RSD)为4.5%。该方法具有所用试剂量少、操作方便、灵敏度高、精密度高、稳定性高等优点,适宜桑椹样品总多酚含量的测定。

关键词: 桑椹, Folin-Ciocaileu, 比色, 总多酚含量

Abstract:

We investigated the optimum reaction conditions of Folin-Ciocalteu colorimetry for the determination of total polyphenos in 60% ethanol extract of ethanol mulberry fruits using gallic acid as a reference. When ratio of Folin-Ciocalteu reagent to 12% sodium carbonate solution (V/V) was 1:2, reaction temperature 20 ℃, and reaction time 2 h, the content of total polyphenols had a good linear relationship with absorbance at 760 nm. The average spiked recovery was 97.63%, with a RSD of 4.5%. This method can give a simple, fast, sensitive, and stable determination of total popyphenols in mulberry fruits.

Key words: mulberry, Folin-Ciocaileu, colorimetry, content of total polyphenols

中图分类号: