食品科学 ›› 2010, Vol. 31 ›› Issue (1): 141-144.doi: 10.7506/spkx1002-6630-201001033

• 生物工程 • 上一篇    下一篇

内蒙古奶豆腐中产胞外多糖乳酸菌的分离筛选

张 雪,李 达,赵玉娟,牛春华,杨贞耐*   

  1. 吉林省农业科学院农产品加工研究中心
  • 收稿日期:2009-02-24 修回日期:2009-08-24 出版日期:2010-01-01 发布日期:2014-05-19
  • 通讯作者: 杨贞耐 E-mail:zyang@cjaas.com
  • 基金资助:

    国家自然科学基金项目(30670057);吉林省科技厅重点项目(20060219);
    农业部现代农业产业技术体系建设专项资金资助项目(nycytx-0502)

Isolation and Screening of Exopolysaccharides-producing Lactic Acid Bacteria from Inner Mongolia Dairy Tofu

ZHANG Xue,LI Da,ZHAO Yu-juan,NIU Chun-hua,YANG Zhen-nai*   

  1. (Center of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China)
  • Received:2009-02-24 Revised:2009-08-24 Online:2010-01-01 Published:2014-05-19
  • Contact: YANG Zhen-nai* E-mail:zyang@cjaas.com

摘要:

从内蒙古牧民自制的传统发酵乳制品“奶豆腐”中分离得到4 株乳酸杆菌。经表型、生理生化和API 鉴定,这4 株菌都是植物乳杆菌,分别为植物乳杆菌C2、C8、C15 和C21。采用负染法观察菌株荚膜多糖产生情况,菌株C8、C15 和C21 都产生荚膜多糖,在普通光学显微镜下荚膜清晰可见。对4 株植物乳杆菌48h 内的生长和产胞外多糖情况进行分析发现,本实验中的4 株植物乳杆菌在发酵48h 内的胞外多糖产量逐渐增加,其中植物乳杆菌C21 的产量最高。

关键词: 胞外多糖, 荚膜多糖, 内蒙古奶豆腐, 植物乳杆菌

Abstract:

Four Lactobacilli stains were isolated and screened from traditional fermented dairy products dairy tofu popular in Inner Mongolia. According to phenotypic, physiological-biochemical and API tests, the strains were all identified as Lactobacillus plantarum and named C2, C8, C15, and C21, respectively. The negative staining method was used to observe their capsules, and the results indicated that except strain C2, all the others produced capsular polysaccharides and the capsules could be seen by using optical microscopy. The 48 h growth and exopolysaccharide-producing ability of the four strains were analyzed. It was found that all these strains exhibited a gradually increasing exopolysaccharides production during 48 h culture, and strain C21 produced the highest amount of exopolysaccharides.

Key words: exopolysaccharide, capsular polysaccharide, Inner Mongolia dairy tofu, Lactobacillus plantarum

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