食品科学 ›› 2010, Vol. 31 ›› Issue (1): 177-180.doi: 10.7506/spkx1002-6630-201001041

• 生物工程 • 上一篇    下一篇

补料分批发酵生产谷胱甘肽的研究

潘亚磊,贺小贤,陈 珊   

  1. 陕西科技大学生命科学与工程学院
  • 收稿日期:2009-03-17 修回日期:2009-08-11 出版日期:2010-01-01 发布日期:2014-05-19
  • 通讯作者: 潘亚磊 E-mail:panyalei588@163.com
  • 基金资助:

    陕西省教育厅自然科学基金项目(08JK233)

Fed-batch Fermentation of Glutathione

PAN Ya-lei,HE Xiao-xian,CHEN Shan   

  1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi an 710021, China
  • Received:2009-03-17 Revised:2009-08-11 Online:2010-01-01 Published:2014-05-19
  • Contact: PAN Ya-lei E-mail:panyalei588@163.com

摘要:

考察5L 发酵罐中分批补加葡萄糖对发酵生产谷胱甘肽(GSH)的影响。采用20g/L 初糖质量浓度,在发酵12h 至27h 每隔3h 分别补加22、24、24、24、24g/L 和22g/L 葡萄糖,可以使酿酒酵母在发酵33h 时GSH 质量浓度达到72.49mg/L,细胞干质量浓度达到28.52g/L,分别为初糖20g/L 分批培养方式的2.86 倍和4.93 倍。补料分批发酵可以明显促进酿酒酵母生长和提高GSH 的合成。

关键词: 分批补料, 发酵, 谷胱甘肽

Abstract:

In order to obtain high glutathione (GSH) yield and dry cell weight, fed-batch fermentation in a 5 L fermentor was adopted for glutathione production by a DL-ethionine, zinc chloride-resistant mutant of Saccharomyces cerevisiae. Strategy for fed-batch feeding of glucose at an initial concentration of 20 g/L was investigated. Results showed that the fed-batch feeding strategy of adding 22, 24, 24, 24, 24 g/L and 22 g/L glucose at intervals of 3 h from 12 to 27 h of fermentation resulted in 72.49 mg/L GSH concentration and 28.52 g/L dry cell weight, which was 2.86 times and 4.93 times as high as those obtained by single batch fermentation. This meant that fed-batch fermentation could remarkably promote strain growth and enhance GSH production.

Key words: fed-batch, fermentation, glutathione

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