食品科学 ›› 2010, Vol. 31 ›› Issue (1): 219-223.doi: 10.7506/spkx1002-6630-201001051

• 营养卫生 • 上一篇    下一篇

阿魏酸体外抗氧化作用研究

赵文红,邓泽元* ,范亚苇,李 静,阮 征   

  1. 南昌大学 食品科学与技术国家重点实验室,南昌大学高等研究院
  • 收稿日期:2009-04-14 修回日期:2009-08-22 出版日期:2010-01-01 发布日期:2014-05-19
  • 通讯作者: 邓泽元 E-mail:dengzy28@yahoo.com.cn
  • 基金资助:

    江西省教育厅2007 年重点实验室基金项目;教育部“长江学者和创新团队发展计划”项目(IRT0540)

Antioxidant Properties of Ferulic Acid in vitro

ZHAO Wen-hong,DENG Ze-yuan*,FAN Ya-wei,LI Jing,RUAN Zheng   

  1. State Key Laboratory of Food Science and Technology, Institute for Advanced Study, Nanchang University,
    Nanchang 330047, China
  • Received:2009-04-14 Revised:2009-08-22 Online:2010-01-01 Published:2014-05-19
  • Contact: DENG Ze-yuan*, E-mail:dengzy28@yahoo.com.cn

摘要:

为研究阿魏酸的抗氧化作用,以分光光度法测定其对红细胞溶血和肝线粒体肿胀的保护作用;用TBARA法测定其对血清自氧化、肝匀浆自发性及Fe2+ 诱导性脂质过氧化的抑制作用;并测定其对·OH、O2·以及DPPH自由基清除能力和对铁离子的还原能力。结果表明:阿魏酸对Fe3+ 具有很强的还原能力,相对还原力是VC 的2倍;能有效清除Fenton 反应生成的·OH 和次黄嘌呤- 黄嘌岭氧化酶系统产生的O2·以及DPPH 自由基,其IC50分别为1.09mmol/L、0.62mmol/L 和23.56μmol/L;对红细胞溶血和血清自氧化均具有很好的保护作用,并能有效的抑制肝匀浆自发性和Fe2+ 诱导性脂质过氧化。这些结果说明阿魏酸具有明显的抗氧化活性。

关键词: 阿魏酸, 体外, 抗氧化, 自由基, 还原

Abstract:

This study aimed to investigate antioxidant activities of ferulic acid (FA) in vitro by evaluation of protective effect against red blood cell (RBC) hemolysis and swelling of liver mitochondrion in rat detected by spectrophotometric method and inhibitory effect against serum autooxidation and spontaneous and H2O2-Fe2+-induced lipid peroxidation in liver homogenate and hydroxyl radical, superoxide anion radical, DPPH radical scavenging and ferric reducing antioxidant power assays. Results showed that FA had stronger reducing power with above 2-fold enhancement when compared with VC, effectively scavenged hydroxyl radicals generated by Fenton reaction, superoxide anion radicals generated in hypoxanthin-xanthine oxidase system and DPPH radicals, with IC50s of 1.09 mmol/L, 0.62 mmol/L and 23.56 μmol/L, respectively, and could provide good protection against RBC hemolysis and serum autooxidation and inhibit significantly spontaneous and H2O2-Fe2+-induced lipid peroxidation in liver homogenate. Thus, it was concluded that FA possessed outstanding anti-oxidant properties.

Key words: ferulic acid(FA), in vitro, anti-oxidation, free radicals, reducing power

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