食品科学 ›› 2010, Vol. 31 ›› Issue (6): 243-247.doi: 10.7506/spkx1002-6630-201006058

• 分析检测 • 上一篇    下一篇

HPLC 法比较测定紫苏属植物种子维生素E含量差异

蔡乾蓉,吴 卫* ,郑有良,杨文婷,徐应文   

  1. 四川农业大学农学院
  • 收稿日期:2009-05-08 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 吴 卫 E-mail:ewuwei@sicau.edu.cn
  • 基金资助:

    四川农业大学青年科技创新基金项目(00131600)

HPLC Analysis of Vitamin E Content in Seeds of Different Species of Genus Perilla L.

CAI Qian-rong,WU Wei*,ZHENG You-liang,YANG Wen-ting,XU Ying-wen   

  1. Agronomy College, Sichuan Agricultural University, Ya an 625014, China
  • Received:2009-05-08 Online:2010-03-15 Published:2010-12-29
  • Contact: WU Wei*, E-mail:ewuwei@sicau.edu.cn

摘要:

采用HPLC 法测定来自中国四川和广东,以及日本和美国等地共29 份紫苏属不同原(变)种种子的VE 含量。结果表明,紫苏属不同原(变)种种子均含丰富的VE。所有供试材料的δ -VE、γ -VE 和α -VE 含量及总含量间均存在极显著差异。其中,VE 总含量变幅在79.90~383.30μg/g 之间,平均值为199.76μg/g;δ -VE、γ -VE 和α -VE 平均含量分别为5.81、190.47μg/g 和3.48μg/g。以来自日本的P06-37 的VE 总含量最高,达383.30μg/g。比较不同原(变)种种子VE 含量结果表明,不同原(变)种间δ -VE、γ -VE 和α -VE 含量及总含量差异未达显著水平。总体上,以白苏的δ -VE、γ -VE 和α -VE 平均含量及总含量均相对较高。

关键词: 紫苏, 种子, 维生素E, HPLC

Abstract:

HPLC method was used to analyze vitamin E content in 29 seed samples of different original species or variants of genus Perilla L. from Sichuan and Guangdong Provinces, China, Japan, Korea, Thailand or U.S.. Results showed that 29 Perilla L. seed samples were all rich in VE. There were extremely significant differences of total and respective contents of δ-VE, γ- VE and α-VE among 29 Perilla L. seed samples. Total VE contents varied in the range of 79.90 to 383.30 μg/g, with an average value of 199.76 μg/g. The average contents of δ-VE, γ-VE and α-VE were 5.81, 190.47 μg/g and 3.48 μg/g, respectively. Among 29 Perilla L. seed samples, P06-37 from Japan exhibited the highest total VE content, reaching up to 383.30 μg/g. No significant differences in total and respective contents of δ-VE, γ-VE and α-VE among seeds of different original species and variants of genus Perilla L. tested were observed and P. frutescens (L.) Britton var. frutescens (baisu) exhibited the highest average values of total and respective contents of δ-VE, γ - VE and α -VE.

Key words: Perilla L., seed, vitamin E, HPLC

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