食品科学 ›› 2010, Vol. 31 ›› Issue (12): 127-131.doi: 10.7506/spkx1002-6630-201012029

• 工艺技术 • 上一篇    下一篇

大孔树脂对野菊花总黄酮吸附分离特性的影响

叶 春,张 婧   

  1. 贵州大学生命科学学院
  • 收稿日期:2009-08-27 修回日期:2009-12-08 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 叶 春 E-mail:yechun126@163.com

Macroporous Resin Adsorption for the Separation of Total Flavonoids from Chrysanthemum indicum L.

YE Chun,ZHANG Jing   

  1. (College of Life Science, Guizhou University, Guiyang 550025, China)
  • Received:2009-08-27 Revised:2009-12-08 Online:2010-06-15 Published:2010-12-29
  • Contact: YE Chun E-mail:yechun126@163.com

摘要:

选择12 种大孔树脂,比较其对野菊花总黄酮的吸附和解吸性能。在此基础上,筛选出较优的3 种大孔树脂,通过研究其静态吸附动力学特性,进一步筛选出适合分离野菊花总黄酮的理想树脂,并考察pH 值和洗脱剂体积分数对其吸附容量和解吸率的影响。结果表明:XDA-1、LSA-21 和AB-8 树脂有较大的吸附容量和较高的解吸率,且其中XDA-1 树脂显著优于另外两种,其吸附容量高达84.1mg/g、解吸率为96.2%,而且具有良好的静态吸附动力学特性,是更为适合吸附分离野菊花总黄酮的理想树脂。XDA-1 树脂吸附分离野菊花总黄酮较合适的上样液pH 值为4~5,洗脱剂为70% 乙醇溶液。

关键词: 野菊花, 黄酮, 大孔树脂, 吸附, 分离

Abstract:

Totally, 12 macroporous resins were tested for their adsorption and desorption performance to total flavonoids from Chrysanthemum indicum L., and 3 of them, namely XDA-1, LSA-21 and AB-8 resins were selected for subsequent studies, which were carried out to investigate the static adsorption and desorption behaviors of the selected resins. Finally, XDA-1 resin was selected to purify crude total flavonoid extract from Chrysanthemum indicum L. due to its higher adsorption capacity (84.1mg/g) and desorption ratio (96.2%) and better static adsorption kinetics than those of LSA-21 and AB-8 resins. The optimum pH for XDA-1 resin adsorption ranged from 4 to 5 and the optimum desorption solvent 70% ethanol.

Key words: Chrysanthemum indicum L., total flavonoids, macroporous resin, adsorption, separation

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