关键词: 爬山虎籽, 原花青素, 提取纯化, 大孔树脂, HPLC

Abstract:

In this study, we focused on the orthogonal array optimization of ethanol extraction of Parthenocissus tricuspidata seed procyanidins, followed by the investigations on macroporous resin purification of the crude procyanidin extract. The optimum conditions for ethanol extraction of Parthenocissus tricuspidata seed procyanidins were determined to be: ethanol concentration 60% (V/V), extraction temperature 70 ℃, extraction time 90 min and solid-to-liquid ratio 1:10 (g/mL); the procyanidin yield was 1.407% under such conditions. It was showed that AB-8 resin was more suitable for procyanidin purification and that the optimum purification conditions were as follows: sample concentration, 4 mg/mL; desorption solvent, 30% ethanol; and desorption flow rate, 2.0 BV/h. The purity of the final product was determined by HPLC to be 88.0%.

Key words: Parthenocissus tricuspidata seeds, proanthocyanidin, extraction and purification, macroporous resin, HPLC