苹果叶多酚的纯化及其抗氧化性研究

doi:10.7506/spkx1002-6630-201020015

食品科学 ›› 2010, Vol. 31 ›› Issue (20): 72-78.doi: 10.7506/spkx1002-6630-201020015

苹果叶多酚的纯化及其抗氧化性研究

徐怀德，王临宾，张立佳

西北农林科技大学食品科学与工程学院

收稿日期:2009-12-30 出版日期:2010-10-25 发布日期:2010-12-29
通讯作者: 徐怀德 E-mail:xuhuaide@yahoo.com.cn

Purification and Antioxidant Activity of Polyphenols from Apple Tree Leaves

XU Huai-de，WANG Lin-bin，ZHANG Li-jia

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

Received:2009-12-30 Online:2010-10-25 Published:2010-12-29
Contact: Xu Huai-De E-mail:xuhuaide@yahoo.com.cn

摘要/Abstract

摘要：

对苹果叶多酚的纯化及其抗氧化性进行研究。采用大孔吸附树脂对苹果叶多酚进行初步纯化、Sephadex LH-20 进一步精制，并进行高效液相分析。结果表明X-5 树脂对苹果叶多酚有较好的吸附解吸效果，X-5 树脂纯化苹果叶多酚的条件为上柱液质量浓度3.658mg/mL、上柱液pH3、吸附流速2.0mL/min；以体积分数40% 乙醇为解吸剂，洗脱流速1.0mL/min，洗脱体积4BV。在此条件下纯化后的苹果叶多酚的纯度从10.07% 提高到38.55%；X-5 树脂对苹果叶多酚的吸附为放热过程，吸附过程符合Langmuir 等温吸附模型和Freundlich 等温吸附模型。经过X-5 树脂纯化后的苹果叶多酚对DPPH 自由基和NO2 － ·的清除能力有所增强；苹果叶中根皮苷的含量为2.45%，粗提物经过X-5 树脂纯化和Sephadex LH-20 精制后得到的苹果叶多酚精制品中根皮苷的含量为47.61%，占制品中总多酚的91.51%。

关键词: 苹果叶, 多酚, 大孔吸附树脂, 抗氧化性, 根皮苷

Abstract:

The preliminary purification of a polyphenols-rich extract from apple tree leaves prepared in our laboratory was carried out using macroporous resin adsorption, followed by further purification and HPLC analysis. Along with this, the preliminarily purified extract was tested for its ability to scavenge DPPH free radicals and nitrite. X-5 resin was the best resin found for adsorbing and desorbing apple tree leaf polyphenols among eight types of resins tested. The optimum adsorption/ desorption conditions of X-5 resin were determined to be: sample concentration, 3.658 mg/mL; sample pH, 3; sample flow rate, 2.0 mL/min; ethanol concentration for desorption, 40%; desoprtion flow rate, 1.0 mL/min; and desorption solvent volume, 4 BV. After the purification under the above conditions, the purity of polyphenols was increased from 10.07% to 38.55%. The adsorption of X-5 resin towards apple tree leaf polyphenols was an exothermic course and accorded with the Langmuir isothermal model and the Freundlich isothermal model. The purified extract presented stronger DPPH free radical and nitrite scavenging capacities than the native extract. The HPLC analysis showed that the content of phlorizin was 2.45% in apple tree leaves and 47.61% in the further purified extract, which accounted for 91.51% of the total polyphenols.

Key words: apple tree leaves, polyphenols, macroporous adsorption resin, antioxidant activities, phlorizin

中图分类号:

TQ243.11

徐怀德，王临宾，张立佳. 苹果叶多酚的纯化及其抗氧化性研究[J]. 食品科学, 2010, 31(20): 72-78.

XU Huai-de，WANG Lin-bin，ZHANG Li-jia. Purification and Antioxidant Activity of Polyphenols from Apple Tree Leaves[J]. FOOD SCIENCE, 2010, 31(20): 72-78.

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苹果叶多酚的纯化及其抗氧化性研究

Purification and Antioxidant Activity of Polyphenols from Apple Tree Leaves

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