食品科学 ›› 2010, Vol. 31 ›› Issue (20): 83-88.doi: 10.7506/spkx1002-6630-201020017

• 工艺技术 • 上一篇    下一篇

响应面分析果胶酶提高人参果出汁率的工艺参数

苏凤贤1 , 2,王晓琴1 , 2,苟亚峰1,樊子豪1,杜双虎1,张芬琴1 , 2 ,*   

  1. 1.河西学院生命科学与工程系
    2.河西学院生态研究所
  • 收稿日期:2010-01-05 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 张芬琴 E-mail:fenqinzh@hxu.edu.cn

Optimization of Technological Parameters for Pectinase Hydrolysis for Improved Pepino Juice Yield Using Response Surface Analysis

SU Feng-xian1,2,WANG Xiao-qin1,2,GOU Ya-feng1,FAN Zi-hao1,DU Shuang-hu1,ZHANG Fen-qin1,2,*   

  1. 1. Department of Life Science and Engineering, Hexi University, Zhangye 734000, China;
    2. Institute of Ecology, Hexi University, Zhangye 734000, China
  • Received:2010-01-05 Online:2010-10-25 Published:2010-12-29
  • Contact: ZHANG Fen-qin1 E-mail:fenqinzh@hxu.edu.cn

摘要:

果胶酶可以促进果胶水解,提高水果的出汁率。运用响应面法和灰色关联分析优化果胶酶影响人参果出汁率的工艺参数,建立相应的回归模型Y= 89.78333 + 0.86250x3 + 0.67917x4 + 0.80937x32 - 0.83125x1x2。方差分析结果表明,酶解时间、果胶酶用量对人参果出汁率影响显著(P < 0.05),而酶解温度、初始pH 值对出汁率无显著影响(P > 0.05)。多元回归分析结果显示,初始pH 值、酶解温度、酶解时间及果胶酶用量与出汁率之间回归模型拟合程度较好,可用于实际生产预测。

关键词: 果胶酶, 出汁率, 二次正交旋转组合设计, 响应面法(RSM), 灰色关联分析

Abstract:

The optimization of four technological parameters for the pectinase hydrolysis was carried out using response surface and grey correlation analyses in order to improve juice yield. A regression model with juice yield (Y) as a function of initial pH (x1), hydrolysis temperature (x2), hydrolysis time (x3) and enzyme load (x4) was set up as follows: Y = 89.78333+0.86250x3+0.67917x4 + 0.80937x32 - 0.83125x1x2. The analysis of variance for the model showed that hydrolysis time and enzyme load significantly affected juice yield (P<0.05) and that the others had no significant effect on juice yield (P>0.05). The multiple regression analysis indicated that the established model had an excellent goodness of fit, suggesting good reliability in predicting the function.

Key words: pectinase, juice yield, quadratic rotary combination design, response surface methodology (RSM), grey correlation analysis

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