食品科学 ›› 2010, Vol. 31 ›› Issue (20): 121-126.doi: 10.7506/spkx1002-6630-201020025

• 工艺技术 • 上一篇    下一篇

高脱乙酰度蛹渣壳聚糖制备工艺优化

赵 维,李建科*   

  1. 陕西师范大学食品工程与营养科学学院
  • 收稿日期:2010-06-30 修回日期:2010-09-07 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 李建科 E-mail:jiankel@snnu.edu.cn
  • 基金资助:

    陕西省“13115”科技创新工程重大科技专项(2009ZDKG-05)

Optimization of the Preparation Process for Silkworm Chrysalis-derived Highly Deacetylated Chitosan

ZHAO Wei,LI Jian-ke*   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
  • Received:2010-06-30 Revised:2010-09-07 Online:2010-10-25 Published:2010-12-29
  • Contact: LI Jian-ke E-mail:jiankel@snnu.edu.cn

摘要:

制备高脱乙酰度蛹渣壳聚糖。在蛹渣壳聚糖制备工艺单因素试验研究的基础上,应用二次回归正交旋转组合设计方法研究无水乙醇浸泡时间、氢氧化钠溶液质量分数、处理温度、处理时间及料液比对壳聚糖脱乙酰度的影响,建立脱乙酰度对5 个试验因素的正交回归模型,通过频率分析法确定蛹渣壳聚糖较优的制备条件范围并得到蛹渣壳聚糖的最佳制备工艺。最佳工艺为:无水乙醇浸泡1.7h、氢氧化钠溶液质量分数44%、处理温度94℃、处理时间9h、料液比1:28(g/mL)、每2h 换碱1 次。在此工艺条件下,壳聚糖的脱乙酰度95.96%、相对分子质量7.45 × 105、产率56.98%、水分含量3.20%、灰分含量0.35%,产品为原白色,外观色泽好,主要指标均达到相关企业标准。

关键词: 蛹渣, 壳聚糖, 高脱乙酰度, 制备工艺

Abstract:

The chitin obtained as light yellow powder from the residue of silkworm chrysalis left after oil and protein extraction was utilized as the start material to prepare highly deacetylated chitosan in the present study. On the basis of single-factor investigations, quadratic orthogonal rotation combination design was employed to investigate the effects of soaking time in absolute ethanol, NaOH concentration, thermal treatment temperature and duration and material/liquid ratio on deacetylation degree of chitosan. An orthogonal regression model for deacetylation degree of chitosan against the above five factors was constructed. Using frequency analysis method, the optimal conditions for the preparation of highly deacetylated chitosan were determined as follows: soaking time in absolute ethanol, 1.7 h; NaOH concentration, 44%; thermal treatment temperature, 94 ℃; treatment time, 9 h; material/liquid ratio (g/mL), 1:28; and replacement frequency of NaOH, once every two hours. Under these conditions, a product with 95.96% deacetylation degree, 7.45×105 relative molecular mass, 56.98% yield, 3.20% moisture content, 0.35% ash content was obtained as natural white chitosan and each of its major quality indexes met the relevant industrial standards.

Key words: silkworm chrysalis residue, chitosan, high deacetylation degree, preparation process

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