食品科学 ›› 2010, Vol. 31 ›› Issue (20): 394-398.doi: 10.7506/spkx1002-6630-201020082

• 分析检测 • 上一篇    下一篇

碱性橙Ⅱ染料快速检测显色剂筛选及显色条件的优化

纪淑娟,张庆欢,沈昳潇   

  1. 沈阳农业大学食品学院
  • 收稿日期:2010-06-23 修回日期:2010-09-14 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 张庆欢 E-mail:zhang-qing-huan@163.com
  • 基金资助:

    辽宁省教育厅重点项目(2009A633)

Screening for Colour Developer and Optimization of Reaction Conditions for Fast Determination of Basic Orange Ⅱ in Foods

JI Shu-juan,ZHANG Qing-huan,SHEN Yi-xiao   

  1. (College of Food Science, Shenyang Agricultural University, Shenyang 110161, China)
  • Received:2010-06-23 Revised:2010-09-14 Online:2010-10-25 Published:2010-12-29
  • Contact: ZHANG Qing-huan E-mail:zhang-qing-huan@163.com

摘要:

利用碱性橙Ⅱ的显色特性,考察碱性橙Ⅱ染料的快速检测的最佳显色条件。通过对不同显色剂显色效果与吸光度变化的观察分析,筛选确定铁氰化钾- 三氯化铁显色体系为最佳显色剂。在此基础上对显色反应的条件进行优化。结果表明,铁氰化钾和三氯化铁的适宜体积配比为铁氰化钾(3g/L):三氯化铁(4.5g/L)=1:1,在755nm 波长处测定吸光度优化最佳条件,适宜反应条件为反应时间20min;反应温度50℃;显色剂用量0.7mL。

关键词: 碱性橙Ⅱ, 比色法, 快速检测

Abstract:

Using the ability of basic orange Ⅱ to develop color, a fast method was presented for the determination of this dye. The optimal conditions for color development were investigated. A reagent system composed of potassium ferricyanide and Iron (Ⅲ) was found to the best color developer through comparative analysis of color developing effects of different color developers and observation of absorbance change, and the optimal ratio of potassium ferricyanide solution (3 g/L) to ferric chloride solution (4.5 g/L) was 1:1. The optimal reaction conditions were determined based on absorbance at 755 nm wavelength as follows: color developer amount, 0.7 mL; and reaction temperature, 50 ℃ for a reaction duration of 20 min.

Key words: basic orange Ⅱ, colorimetry, ast determination

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