超高效液相色谱测定蜂蜜及其制品中的苯甲酸、山梨酸

doi:10.7506/spkx1002-6630-201020088

食品科学 ›› 2010, Vol. 31 ›› Issue (20): 417-420.doi: 10.7506/spkx1002-6630-201020088

超高效液相色谱测定蜂蜜及其制品中的苯甲酸、山梨酸

黄京平^1,2，李熠^1,2，周金慧^1,2，吴黎明^1,2，陈兰珍^1,2，赵静^1,2，薛晓锋^1,2,*

1.中国农业科学院蜜蜂研究所 2. 农业部国家蜂产品加工专业分中心

收稿日期:2010-05-13 出版日期:2010-10-25 发布日期:2010-12-29
通讯作者: 薛晓锋 E-mail:xue_xiaofeng@126.com
基金资助:
国家现代农业(峰)产业技术体系项目(NYCYTX-43)

Determination of Benzoic Acid and Sorbic Acid in Honey and Honey Products by Ultra-performance Liquid Chromatography

HUANG Jing-ping1,2，LI Yi1,2，ZHOU Jin-hui1,2，WU Li-ming1,2，CHEN Lan-zhen1,2，ZHAO Jing1,2，XUE Xiao-feng1,2,*

1. Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China；
2. National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China

Received:2010-05-13 Online:2010-10-25 Published:2010-12-29
Contact: XUE Xiao-feng1 E-mail:xue_xiaofeng@126.com

摘要/Abstract

摘要：

研究蜂蜜及其制品中的山梨酸与苯甲酸两种防腐剂的提取净化及其超高效液相色谱检测方法。样品用水溶解，超声波辅助提取样品中的山梨酸与苯甲酸。样液经高速离心，滤膜过滤后，采用超高效液相色谱梯度洗脱与二极管阵列检测器测定样品中的山梨酸与苯甲酸的含量。以BEH Shield RP18(2.1mm × 100mm，1.7μm)为色谱柱，以0.02mol/L 乙酸铵溶液和乙醇为流动相梯度洗脱，两种防腐剂在4min 中内实现较好的分离。方法检出限：苯甲酸为2.5mg/kg，山梨酸为2.0mg/kg。以加标样品做添加回收率测定，苯甲酸与山梨酸的回收率分别为94.1%～102.3% 和96.2%～99.8%，相对标准偏差均小于5%。方法简单、快速准确、灵敏度高是一种较好的定性和定量方法。

关键词: 蜂蜜, 蜂蜜制品, 苯甲酸, 山梨酸, 超高效液相色谱

Abstract:

In this article, extraction, clean-up and ultra-performance liquid chromatographic analysis of benzoic acid and sorbic acid in honey and honey products were investigated. The samples were dissolved in water first. Benzoic acid and sorbic acid were extracted by an ultrasonic method. After clean up processing with high-speed centrifugation and filtration, the extract was determined by an ultra-performance liquid chromatography with PDA detector. A gradient separation was achieved within 4 min using BEH Shield RP18 column (2.1 mm ×100 mm, 1.7 μm) with ammonium acetate and ethanol as a mobile phase. The developed method was a simple, fast and sensitive. The limit of detection for benzoic acid and sorbic acid were 2.5 mg/kg and 2.0 mg/kg, respectively. The recovery rates of spiked benzoic acid and sorbic acid were 94.1%－102.3% and 96.2%－99.8%, respectively. The relative standard deviations were less than 5%. This method has been successfully applied to analyze some real samples from beekeeper and market for investigating the distribution of benzoic acid and sorbic acid in honey and honey products.

Key words: honey, honey product, benzoic acid, sorbic acid, ultra-performance liquid chromatography

中图分类号:

TS207.3

黄京平1,2，李熠1,2，周金慧1,2，吴黎明1,2，陈兰珍1,2，赵静1,2，薛晓锋1,2,*. 超高效液相色谱测定蜂蜜及其制品中的苯甲酸、山梨酸[J]. 食品科学, 2010, 31(20): 417-420.

HUANG Jing-ping1,2，LI Yi1,2，ZHOU Jin-hui1,2，WU Li-ming1,2，CHEN Lan-zhen1,2，ZHAO Jing1,2，XUE Xiao-feng1,2,*. Determination of Benzoic Acid and Sorbic Acid in Honey and Honey Products by Ultra-performance Liquid Chromatography[J]. FOOD SCIENCE, 2010, 31(20): 417-420.

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超高效液相色谱测定蜂蜜及其制品中的苯甲酸、山梨酸

Determination of Benzoic Acid and Sorbic Acid in Honey and Honey Products by Ultra-performance Liquid Chromatography

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