食品科学 ›› 2010, Vol. 31 ›› Issue (20): 464-468.doi: 10.7506/spkx1002-6630-201020098

• 包装贮运 • 上一篇    下一篇

南极磷虾冷藏过程中的品质变化

李学英1,迟 海1,2,杨宪时1,*,许 钟1,郭全友1   

  1. 1.中国水产科学研究院东海水产研究所
    2.大连海洋大学食品工程学院
  • 收稿日期:2010-06-12 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 杨宪时 E-mail:xianshiyang@126.com
  • 基金资助:

    中央级公益性科研院所基本科研业务费专项(2009T05);农业部公益性行业科研专项(201003042)

Quality Change of Antarctic Krill during Chilling Storage

LI Xue-ying1,CHI Hai1,2,YANG Xian-shi1,*,XU Zhong1,GUO Quan-you1   

  1. (1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
    2. College of Food Engineering, Dalian Ocean University, Dalian 116023, China)
  • Received:2010-06-12 Online:2010-10-25 Published:2010-12-29
  • Contact: YANG Xian-shi E-mail:xianshiyang@126.com

摘要:

以pH 值、出肉率、可溶性蛋白质含量为指标,结合感官评价,探讨了南极磷虾0℃和3℃贮藏过程中的品质变化,运用SDS- 聚丙烯酰胺凝胶电泳(SDS-PAGE)分析南极磷虾的蛋白质相对分子质量变化。实验结果显示,南极磷虾24h 贮藏后,pH 值都逐渐升高,分别达到8.21 ± 0.01 和8.28 ± 0.02;出肉率从(61.92 ± 2.50)% 分别减少到(22.65 ± 6.81)% 和(13.64 ± 2.72)%;可溶性蛋白质含量都逐渐降低,分别降为(19.0 ± 0.50)mg/10g 湿质量和(25.5 ± 2.00)mg/10g 湿质量。SDS- 聚丙烯酰胺凝胶电泳出现5 条蛋白质条带,1h 时0℃和3℃的蛋白质分子质量分别为49.5、32、29、26、13.2kD,且29kD 的蛋白质条带被分解的最快。感官评分最高值和最低值分别为9.5 ± 0.05、9.4 ± 0.12 和1.3 ± 0.10、1.0 ± 0.08。表明在0℃和3℃条件下贮藏的南极磷虾自溶迅速,品质下降很快,因此冷藏条件下的南极磷虾开始进行加工的时间应该在10h 之内。

关键词: 南极磷虾, 自溶, 品质变化

Abstract:

The quality change of Antarctic krill during the storage at 0 ℃ and 3 ℃ was evaluated by pH, meat productivity, soluble protein content and sensory evaluation. Meanwhile, the change in relative molecular mass of proteins from Antarctic krill was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results indicated that pH in Antarctic krill exhibited a gradual increase during the period of storage for 24 h at 0 ℃ and 3 ℃, which were 8.21 ± 0.01 and 8.28 ±0.02, respectively. The meat productivity was decreased from (61.92 ± 2.50)% to (22.65 ± 6.81)% and (13.64 ± 2.72)%, respectively. Soluble protein content also exhibited a decrease and reduced to (19.0 ± 0.50) mg/(10 g wet weight) and (25.5 ±2.00) mg/(10 g wet weight), respectively. Totally 5 protein bands after 1-h storage at 0 ℃ and 3 ℃ was shown in the SDS-PAGE, which had molecular weights of 49.5, 32, 29, 26 kD and 13.2 kD, respectively. However, the protein with molecular weights of 29 kD exhibited the fastest degradation. The highest and lowest values of sensory evaluation were 9.5 ± 0.05, 9.4 ± 0.12, 1.3 ±0.10 and 1.0 ± 0.08, respectively. All of these investigations suggested that the quality of Antarctic krill could be quickly decreased due to its autolysis during storage at 0 ℃ and 3 ℃. Therefore, in order to achieve the best quality and edible properties of Antarctic krill, the optimal processing should be completed within 10 hours at 0 ℃ and 3 ℃ during storage.

Key words: Antarctic krill, autolysis, quality change

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