Fe3O4/PSF磁性复合超滤膜分离麦冬多糖

doi:10.7506/spkx1002-6630-201022009

食品科学 ›› 2010, Vol. 31 ›› Issue (22): 41-45.doi: 10.7506/spkx1002-6630-201022009

Fe3O4/PSF磁性复合超滤膜分离麦冬多糖

谢慧明，李超，潘见，张仕发，伍志刚，朱莹莹

合肥工业大学农产品生物化工教育部工程研究中心

收稿日期:2009-12-28 修回日期:2010-05-22 出版日期:2010-11-25 发布日期:2010-12-29
通讯作者: 谢慧明 E-mail:panxie@163.com
基金资助:
国家“863”计划项目(2007AA100404)

Using Fe3O4-PSF Magnetic Composite Ultrafiltration Membrane for the Separation of Ophiopogon japonicus Polysaccharides

XIE Hui-ming，LI Chao，PAN Jian，ZHANG Shi-fa，WU Zhi-gang，ZHU Ying-ying

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China

Received:2009-12-28 Revised:2010-05-22 Online:2010-11-25 Published:2010-12-29
Contact: XIE Hui-ming E-mail:panxie@163.com

摘要/Abstract

摘要：

以原位生成法制得Fe3O4-PSF(聚砜)磁性复合超滤膜。改变磁场强度和压力，以截留率为考察指标，确定膜截留分子质量为30000D 和10000D 时对应的值分别为0.1T、0.4MPa 和0.8T、0.5MPa。对麦冬多糖提取液(含量为91.68%)进行连续性分离，并对分离后样品进行测定，GPC 软件分析A、C、D 3 样品结果：A 样品Mw 为32610D，分子质量30000D 以上的麦冬多糖的含量85.6%；C 样品Mw 为24069，分子质量10000～30000D 的麦冬多糖的含量达87.6%；D 样品Mw 为8664，分子质量1000～10000D 的麦冬多糖含量达88.7%。

关键词: Fe3O4/PSF磁性复合超滤膜, 麦冬多糖, 连续性分离, 超滤, 高效凝胶液相色谱

Abstract:

A Fe3O4-PSF magnetic composite ultrafiltration membrane with a molecular weight cutoff (MWCO) of 33000 D was obtained by in-situ generation method. The effects of magnetic intensity and ultrafiltration pressure on retention rate were dealt with under MWCOs of 30000 and 10000 D, and it was found that the optimal magnetic intensity and ultrafiltration pressure were 0.1 T and 0.4 MPa for 30000 D MWCO ultrafiltration and 0.8 T and 0.5 MPa for 10000 D MWCO ultrafiltration, respectively. The polysaccharide-rich extract from Ophiopogon japonicus prepared in this study was continuously separated using the two MWCOs, and the fractions (A, C and D) obtained were subjected to the determination of molecular weight based on the use of the GPC software. Fraction A exhibited a molecular weight of 32610 D, and contained 85.6% polysaccharides having a molecular weight exceeding 30000 D. The molecular weight of fraction C was 24069 D, and it contained as high as 87.6% polysaccharides having a molecular weight varying from 10000 to 30000 D. Fraction D had a molecular weight of 8664 D, and the content of polysaccharides having a molecular weight varying from 1000 to 10000 D in it was as high as 88.7%.

Key words: Fe3O4-PSF magnetic composite ultrafiltration membrane, Ophiopogon japonicus polysaccharide, continuous separation, utrafiltration, HPGPC

中图分类号:

R284.2

谢慧明，李超，潘见，张仕发，伍志刚，朱莹莹. Fe3O4/PSF磁性复合超滤膜分离麦冬多糖[J]. 食品科学, 2010, 31(22): 41-45.

XIE Hui-ming，LI Chao，PAN Jian，ZHANG Shi-fa，WU Zhi-gang，ZHU Ying-ying. Using Fe3O4-PSF Magnetic Composite Ultrafiltration Membrane for the Separation of Ophiopogon japonicus Polysaccharides[J]. FOOD SCIENCE, 2010, 31(22): 41-45.

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Fe3O4/PSF磁性复合超滤膜分离麦冬多糖

Using Fe3O4-PSF Magnetic Composite Ultrafiltration Membrane for the Separation of Ophiopogon japonicus Polysaccharides

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