食品科学 ›› 2011, Vol. 32 ›› Issue (4): 155-158.doi: 10.7506/spkx1002-6630-201104033

• 分析检测 • 上一篇    下一篇

软枣猕猴桃总黄酮含量测定方法研究

栾云峰,王 菲,刘长江,宗绪岩   

  1. 沈阳农业大学食品学院
  • 出版日期:2011-03-12 发布日期:2017-04-06
  • 基金资助:
    农业部公益性行业科研专项(200903013)

A Comparative Study of Different Methods for the Determination of Total Flavonids in Actinidia arguta

LUAN Yun-feng,WANG Fei,LIU Chang-jiang,ZONG Xu-yan   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Online:2011-03-12 Published:2017-04-06

摘要: 选用ZrOCl2比色法、NaNO2-Al(NO3)3比色法、AlCl3比色法以及HPLC法测定软枣猕猴桃总黄酮含量,通过对黄酮粗提液和芦丁标准品与不同显色剂反应后进行200~600nm波长扫描,根据扫描结果确定各种反应的适合波长并进行定量分析比较。结果表明,NaNO2-Al(NO3)3比色法与AlCl3比色法不适合软枣猕猴桃总黄酮测定。在284nm波长处,ZrOCl2比色法的线性方程Y=0.0114X-0.0001,R2=0.9991,平均加样回收率为99.4%,RSD=1.61%(n=5)。ZrOCl2比色法是一种快捷、准确的检测方法,适用于软枣猕猴桃总黄酮含量测定。

关键词: 软枣猕猴桃, 总黄酮, 比色法

Abstract: Total flavonoids in Actinidia arguta were determined separately by ZrOCl2 colorimetry, NaNO2-Al(NO3)3 colorimetry, AlCl3 colorimetry and HPLC. Wavelength scanning of flavonoid extracts and rutin after reaction with different chromogenic reagents was performed in a range of 200-600 nm. Results showed that NaNO2-Al(NO3)3 colorimetry and AlCl3 colorimetry were fount not suitable for the determination of total flavonoids in Actinidia arguta. An excellent linear equation for ZrOCl2 colorimetric determination at 284 nm wavelength was obtained as follows: Y284 = 0.0114X-0.0001, R2 = 0.9991. The average recovery rate was 99.4% with a RSD of 1.61% (n = 5). ZrOCl2 colorimetry is a quick accurate method for the determination of total flavonoids in Actinidia arguta.

Key words: Actinidia arguta, total flavonoids, colorimetry

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