多穗柯三叶苷的抑制糖尿病关键酶活性和抗氧化性

doi:10.7506/spkx1002-6630-201105007

食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 32-35.doi: 10.7506/spkx1002-6630-201105007

多穗柯三叶苷的抑制糖尿病关键酶活性和抗氧化性

张毅¹,宁正祥¹,董华强²

1. 华南理工大学轻工与食品学院
2. 佛山科学技术学院食品科学系

收稿日期:2010-07-06 修回日期:2011-01-17 出版日期:2011-03-15 发布日期:2011-03-03
通讯作者: 张毅 E-mail:zhangyi85518@yahoo.com.cn
基金资助:
广东省自然科学基金项目(8152800001000023)

Inhibitory Potential of Trilobatin from Lithocarpus polystachyus Rehd against Key Enzymes Linked to Type Ⅱ Diabetes and Its Antioxidant Activity

ZHANG Yi1，NING Zheng-xiang1,*，DONG Huang-qiang2

1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China；
2. Department of Food Science, Foshan University, Foshan 528231, China

Received:2010-07-06 Revised:2011-01-17 Online:2011-03-15 Published:2011-03-03
Contact: ZHANG Yi E-mail:zhangyi85518@yahoo.com.cn

摘要/Abstract

摘要： 从多穗柯中提取分离得到三叶苷，以抗糖尿病药物阿卡波糖为阳性对照，检测多穗柯三叶苷对小肠α-葡萄糖苷酶和胰脏α-淀粉酶活性的抑制作用和对α-葡萄糖苷酶活性抑制类型，并以芦丁为阳性对照检测其清除DPPH自由基能力。结果显示：多穗柯三叶苷对α-葡萄糖苷酶有明显抑制作用，为非竞争性抑制，抑制效果与阿卡波糖无明显差别，对α-淀粉酶活性的抑制率低于阿卡波糖；清除DPPH自由基能力强于芦丁。表明多穗柯三叶苷是一种有应用潜力的抗糖尿病活性物质。

关键词: 三叶苷, 多穗柯, α-葡萄糖苷酶, α-淀粉酶, 糖尿病

Abstract: The inhibitory effects of trilobatin separated from Lithocarpus polystachyus Rehd on the activities of α-glucosidase and α-amylase were determined and compared with those of the anti-diabetic drug acarbose as a positive control and the type of the enzymatic inhibition on α-amylase was investigated. Also, rutin was used as a positive control to measure the ability of the trilobatin from Lithocarpus polystachyus Rehd to scavenge DPPH free radicals. The results showed that α-glucosidase activity was inhibited remarkably by the trilobatin, and the inhibition was noncompetitive and did not exhibit a significant difference when compared to acarbose. Its inhibition rate against α-amylase, however, was lower than that of acarbose despite of having stronger DPPH free radical scavenging effect than rutin. From these experimental results, a conclusion could be drawn that the trilobatin from Lithocarpus polystachyus Rehd is a potential anti-diabetic compound.

Key words: trilobatin, Lithocarpus polystachyus Rehd, α-glucosidase, α-amylase, diabetes

中图分类号:

Q946.83

张毅1，宁正祥1,*，董华强2. 多穗柯三叶苷的抑制糖尿病关键酶活性和抗氧化性[J]. 食品科学, 2011, 32(5 ): 32-35.

ZHANG Yi1，NING Zheng-xiang1,*，DONG Huang-qiang2. Inhibitory Potential of Trilobatin from Lithocarpus polystachyus Rehd against Key Enzymes Linked to Type Ⅱ Diabetes and Its Antioxidant Activity[J]. FOOD SCIENCE, 2011, 32(5 ): 32-35.

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多穗柯三叶苷的抑制糖尿病关键酶活性和抗氧化性

Inhibitory Potential of Trilobatin from Lithocarpus polystachyus Rehd against Key Enzymes Linked to Type Ⅱ Diabetes and Its Antioxidant Activity

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