食品科学 ›› 2011, Vol. 32 ›› Issue (11): 334-339.doi: 10.7506/spkx1002-6630-201111070

• 专题论述 • 上一篇    

食品中微生物源毒素检测方法研究进展

李晓蓓,欧 杰*,王 婧   

  1. 上海海洋大学食品学院
  • 出版日期:2011-06-15 发布日期:2011-05-13
  • 基金资助:
    上海市科委重点科技攻关项目(10391902300)

A Review of Detection Methods for Microbial Toxins in Foods

LI Xiao-bei,OU Jie*,WANG Jing   

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2011-06-15 Published:2011-05-13

摘要: 食品中的微生物源毒素是指由微生物代谢产生的毒素,主要包括肽类毒素、脂多糖内毒素、霉菌毒素、生物胺和脂肪酸氧化产生的醛类化合物等。通过介绍国内外学者对这几种毒素的检测方法,在对聚合酶链反应技术、酶联免疫检测技术、高效液相色谱法、固相微萃取-气相色谱-质谱法和固相微萃取-气相色谱-嗅觉法等检测方法综合分析的基础上,评述上述方法的特点和适用条件,并总结食品中的微生物源毒素检测技术的研究进展。

关键词: 霉菌毒素, 检测方法, 肽类毒素, 生物胺, 脂多糖内毒素, 醛类化合物

Abstract: Microbial toxin in food products usually refers to the toxin produced by microbial metabolism. It includes peptide toxins, lipopolysaccharide (LPS), mycotoxins, biogenic amines and aldehyde compounds from fatty acid oxidation. This paper briefly introduces partial detection technologies at home and abroad, including polymerase chain reaction (PCR), enzyme linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC), solid-phase microextraction-gas chromatography-mass (SPME-GC-MS), solid-phase microextracton-gas chromatography-olfactometry (SPME-GC-O), etc. The detection technologies are comprehensively evaluated for their features and suitable conditions. Moreover, the recent progress of the research is summarized on their application for detecting several common microbial toxins.

Key words: peptidetoxins, lipopolysaccharide, mycotoxins, biogenic amines, aldehyde compound, detection methods

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