食品科学 ›› 2011, Vol. 32 ›› Issue (14): 181-184.doi: 10.7506/spkx1002-6630-201114037

• 分析检测 • 上一篇    下一篇

HPLC-UV法测定微生物降解体系中3-苯氧基苯甲酸含量

赵 楠,刘书亮*,赖 文,袁怀瑜   

  1. 四川农业大学食品学院
  • 出版日期:2011-07-25 发布日期:2011-06-18
  • 基金资助:
    国家自然科学基金面上项目(21072137)

Determination of 3-Phenoxybenzoic Acid in Microbial Degradation Systems by HPLC-UV Detection

ZHAO Nan,LIU Shu-liang*,LAI Wen,YUAN Huai-yu   

  1. (College of Food Science, Sichuan Agricultural University, Ya’an 625014, China)
  • Online:2011-07-25 Published:2011-06-18

摘要: 采用高效液相色谱-紫外(high-performance liquid chromatography with ultraviolet,HPLC-UV)法测定微生物降解体系中3-苯氧基苯甲酸(3-PBA)的含量。以Gemini 100A C18柱(150mm×4.60mm,5.0μm)为色谱柱,乙腈-水(70:30,V/V)为流动相,流速0.7mL/min,用紫外检测器在210nm处检测3-PBA。结果显示:3-PBA对照品的保留时间为4.268min,线性范围为0.5~50.0mg/L,3-PBA平均回收率为98.919%,RSD为2.78%;运用该方法测得4种不同来源的混合菌株发酵液中3-PBA的残留量分别为24.467、86.266、2.633、1.921mg/L。本法简单、快速、准确、分离度好。

关键词: 检测, HPLC-UV法, 3-苯氧基苯甲酸(3-PBA), 微生物降解体系

Abstract: A HPLC method with UV detection was proposed to determine the concentration of 3-phenoxybenzoic acid (3-PBA) in microbial degradation systems. The chromatographic separation was performed on a Gemini 100A C18 column (5.0μm, 150 mm × 4.60 mm, i.d.) using a mobile phase made up of acetonitrile and water (70:30, V/V) at a flow rate of 0.7 mL/min, and 3-PBA was detected at 210 nm using a UV detector. The retention time of 3-PBA was 4.268 min. The linear range of the method was 0.5-50.0 mg/L. The average spike recovery for 3-PBA was 98.919%, with a relative standard deviation (RSD) of 2.78%. The residues of 3-PBA in 4 different microbial degradation systems were 24.467, 86.266, 2.633 mg/L and 1.921 mg/L, respectively. The developed method demonstrated the benefits of simplicity, rapidity, high accuracy and good separation.

Key words: HPLC-UV, 3-phenoxybenzoic acid, microbial degradation system, determination

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