食品科学 ›› 2011, Vol. 32 ›› Issue (18): 19-23.doi: 10.7506/spkx1002-6630-201118005

• 工艺技术 • 上一篇    下一篇

巴旦木蛋白提取工艺

孙月娥1,2,明 鸣1,王卫东1,高明侠1   

  1. 1.徐州工程学院食品学院 2.江苏省食品生物加工工程技术研究中心
  • 出版日期:2011-09-25 发布日期:2011-09-24
  • 基金资助:
    苏北科技发展计划项目(BN2010005);徐州工程学院培育项目(XKY2009216)

Optimization of Protein Extraction from Amygdalus communis L. Seed Kernel

SUN Yue-e1,2,MING Ming1,WANG Wei-dong1,GAO Ming-xia1   

  1. (1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221111, China ; 2. Jiangsu Engineering Research Center for Food Biology Processing, Xuzhou 221111, China)
  • Online:2011-09-25 Published:2011-09-24

摘要: 以巴旦木为原料,采用碱提酸沉法和超声波辅助碱液浸提法制备巴旦木蛋白,并对其理化性质进行研究。结果表明:碱提酸沉法提取巴旦木蛋白的最佳工艺条件是料液比1:25、pH10、60℃浸提40min;超声辅助碱液浸提法提取巴旦木蛋白的最佳工艺条件是pH9.0、料液比1:20、提取时间15min、超声功率125kW,在此条件下提取率为37.16%。与碱提酸沉法比较,超声波辅助碱液提取法时间短、得率高。

关键词: 巴旦木, 蛋白, 提取, 性质

Abstract: Orthogonal array design was used to optimize the extraction of protein from almond (Amygdalus communis L.) seed kernel by alkaline extraction and acid precipitation method or ultrasonic-assisted alkaline extraction method. The optimal conditions for protein extraction by alkaline extraction and acid precipitation were obtained as follows: material/liquid ratio 1:25, pH 10.0, extraction time 40 min, and extraction temperature 60 ℃. The optimal ultrasonic-assisted alkaline extraction conditions were material/liquid ratio 1:20, pH 9.0, extraction time 15 min, and ultrasonic power 125 kW, resulting in an extraction efficiency of 37.16%. Therefore, the ultrasonic-assisted alkaline extraction method revealed the advantages of shorter extraction time and higher extraction efficiency over the alkaline extraction and acid precipitation method.

Key words: Amygdalus communis L., protein, extraction, properties

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