食品科学 ›› 2011, Vol. 32 ›› Issue (18): 128-132.doi: 10.7506/spkx1002-6630-201118027

• 工艺技术 • 上一篇    下一篇

响应面分析法优化果胶提取工艺

薛照辉1,张 鑫1,张志军2,刘建华2,汪一帆1,陈冬旭1,龙利胜1   

  1. 1. 天津大学化工学院食品科学系
    2.天津市林业果树研究所
  • 出版日期:2011-09-25 发布日期:2011-09-24
  • 基金资助:
    天津大学自主创新基金项目(60302010)

Optimization of Pectin Extraction from Citrus Peel by Response Surface Methodology

XUE Zhao-hui1,ZHANG Xin1,ZHANG Zhi-jun2,LIU Jian-hua2,WANG Yi-fan1,CHEN Dong-xu1,LONG Li-sheng1   

  1. (1. Department of Food Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 2. Tianjin Forestry and Fruit Tree Institute, Tianjin 300112, China)
  • Online:2011-09-25 Published:2011-09-24

摘要: 以干柑橘皮为原料,采用酸提醇沉法对其果胶进行提取。通过单因素、响应面分析法等设计系统研究料液比、提取温度、时间、pH值等影响果胶得率的因素,对果胶提取条件进行优化和显著性分析,从而确定果胶提取的最佳条件为液料比19.7:1、提取温度87.5、提取时间84min、pH1.51。在最优条件下提取果胶的得率为7.294%,与预期值(7.316%)相差不大,因而优化的组合可用于指导产业化制备果胶。

关键词: 响应曲面法, 柑橘, 果胶, 提取

Abstract: Acid extraction followed by ethanol precipitation was used to extract pectin from citrus peel in the present work. Four factors that affect the extraction yield of pectin including material-to-liquid ratio, temperature, extraction time and pH were systematically investigated by one-factor-at-a-time method and response surface analysis. Further, the four factors were optimized and their significance was analyzed. As a result, the optimal conditions for pectin extraction were determined as follows: material-to-liquid ratio 19.7:1, temperature 87.5 ℃, extraction time 84 min, and pH 1.51. Under these conditions, the actual pectin yield was 7.294%, which was close to the predicted value of 7.316%. Thus, the optimized process can provide useful guidance for industrial pectin preparation.

Key words: response surface methodology (RSM), citrus peel, pectin, extraction

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