食品科学 ›› 2011, Vol. 32 ›› Issue (18): 168-170.doi: 10.7506/spkx1002-6630-201118035

• 分析检测 • 上一篇    下一篇

胡柚中3种主要黄酮类物质的含量测定

吴 倩,朴香兰*,杨 静   

  1. 中央民族大学 中国少数民族传统医学研究院
  • 出版日期:2011-09-25 发布日期:2011-09-24
  • 基金资助:
    国家自然科学基金面上基金项目(30973960);国家自然科学基金中韩合作研究项目(81011140347); 国家民委科研项目(09ZY17)

Determination of Three Major Flavonoids in Citrus paradisi cv. Changshan Huyou Fruit

WU Qian,PIAO Xiang-lan*,YANG Jing   

  1. (Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China)
  • Online:2011-09-25 Published:2011-09-24

摘要: 目的:建立常山胡柚3种主要黄酮类物质含量的测定方法,并比较常山胡柚中皮、肉、籽中该3种黄酮类物质含量的差异。方法:采用反相高效液相色谱法分离检测胡柚中柚皮苷、橙皮苷、新橙皮苷的含量;流动相为乙腈-水(23:77,V/V),柱温25℃,流速1.0mL/min,检测波长283nm。结果:柚皮苷在3.22~1030μg/mL、橙皮苷在0.59~380μg/mL、新橙皮苷在0.077~990μg/mL范围内,黄酮类物质的质量浓度与峰面积呈良好的线性关系;柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为98.43%(RSD=4.07%)、98.70%(RSD=4.30%)、99.90%(RSD=3.47%);并以柚皮苷、橙皮苷和新橙皮苷为标准比较胡柚副产物皮、籽与胡柚肉含量的差异,初步得出皮>肉>籽。结论:该方法简便、准确、可靠、重复性好,可用于常山胡柚的质量控制。

关键词: 胡柚, 柚皮苷, 橙皮苷, 新橙皮苷

Abstract: Objective: To establish a method for determining naringin, hesperidin, and neohesperindin in Citrus paradisi cv. Changshan Huyou fruit and compare their contents in different parts such as peel, pulp and seeds. Methods: The contents of naringin, hesperidin and neohesperindin were determined by a reversed-phase HPLC method. Methanol extracts of samples and the standard substances were separated on a YMC C18 column (4.6 mm × 250 mm, 5μm) using a mobile phase made up of acetonitrile and water (23:77 , V/V) at a flow rate of 1 mL/min and detected by a UV detector at a wavelength of 283 nm. The column temperature was set at 25 ℃. Results: The calibration curves were linear in the range of 3.22-1030μg/mL for naringin, 0.59-380μg/mL for hesperidin, and 0.077-990μg/m for neohesperindin, respectively. The average recoveries of naringin, hesperidin and neohesperindin were 98.43% (RSD = 4.07%), 98.70% (RSD = 4.30%) and 99.90% (RSD = 3.47%), respectively. The contents of naringin, hesperidin, neohesperindin were the highest in the peel and the lowest in the seeds of Citrus paradisi cv. Changshan Huyou fruit. Conclusions: This method is simple, accurate, reliable and reproducible and can therefore be used for the quality control of Citrus paradisi cv. Changshan Huyou fruit.

Key words: Citrus paradisi cv. Changshan Huyou, naringin, hesperidin, neohesperindin, determination

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