食品科学 ›› 2011, Vol. 32 ›› Issue (19): 16-22.doi: 10.7506/spkx1002-6630-201119004

• 基础研究 • 上一篇    下一篇

金荞麦乙酸乙酯萃取物化学成分的分离鉴定

赵利琴,张小平,*,张朝凤2   

  1. 1.安徽师范大学 安徽省重要生物资源保护与利用研究重点实验室 2.中国药科大学生药学研究室
  • 发布日期:2011-10-12
  • 基金资助:
    国家自然科学基金项目(307000060)

Fractionation and Identification of Ethyl Ecetate Extract from Rhizoma Fagopyri Cymosi

ZHAO Li-qin1,2,ZHANG Xiao-ping1,*,ZHANG Chao-feng2   

  1. (1. Key Laboratory of Conservation and Employment of Biological Resources, Anhui Normal University, Wuhu 241000, China ; 2. Research Department of Pharmacagnosy, China Pharmaceutical University, Nanjing 211198, China)
  • Published:2011-10-12

摘要: 目的:对蓼科荞麦属植物金荞麦的化学成分进行分离鉴定。方法:采用硅胶色谱、凝胶色谱和重结晶等技术进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果:分离纯化了13个化合物,通过波谱鉴定分别为:赤杨酮(化合物1)、异鼠李素(化合物2)、槲皮素(化合物3)、槲皮素-3-O-鼠李糖苷(化合物4)、圣草酚(化合物5)、儿茶素(化合物6)、3,5-二甲氧基苯甲酸-4-O-葡萄糖苷(化合物7)、丁香酸(化合物8)、对羟基苯甲醛(化合物9)、琥珀酸(化合物10)、N-反式香豆酰酪胺(化合物11)、β-谷甾醇(化合物12) 和胡萝卜苷(化合物13)。结论:圣草酚、3,5-二甲氧基苯甲酸-4-O-葡萄糖苷和N-反式香豆酰酪胺是在金荞麦属内首次发现,丁香酸是种内首次发现。

关键词: 金荞麦, 化学成分, 分离, 鉴定

Abstract: Objective: To fractionate the ethyl ecetate extract from Rhizoma Fagopyri Cymosi and identify the resulting fractions. Methods: Silica gel column chromatography, Sephadex LH-20 column chromatography and recrystailization were employed to fractionate the ethyl ecetate extract. According to physiochemical properties and spectral data, the fractions obtained were identified. Results: Thirteen compounds were separated from the ethyl ecetate extract and elucidated as glutinone (1), isorhametin (2), quercetin (3), quercetin-3-O-α-L-rhamnoside (4), eriodictyol (5), catechin (6), 3,5-dimethoxy benzene carbonic acid-4-O-glucoside (7), syringic acid (8), p-hydroxyl-benzaldehyde (9), succinic acid (10), N-trans-coumaroyl tyramine (11), sitosterol (12) and daucosterol (13). Conclusion: For the first time, compounds 5, 7 and 11 have been isolated from the genus of Fagopyrum, and compound 8 from Fagopyrum dibotrys (D.Don) Hara.

Key words: Fagopyrum dibotrys, chemical composition, isolation, identification

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