苦荞多糖的分离纯化及单糖组成测定

doi:10.7506/spkx1002-6630-201119007

食品科学 ›› 2011, Vol. 32 ›› Issue (19): 33-36.doi: 10.7506/spkx1002-6630-201119007

苦荞多糖的分离纯化及单糖组成测定

颜军，孙晓春，谢贞建，何钢，易勇，房晨，苟小军

1.成都大学中药化学实验室 2.四川抗菌素工业研究所

发布日期:2011-10-12
基金资助:
四川省中医药管理局科技专项资助项目(2008-01)；四川省教育厅科研项目资助项目(08ZA173)；成都大学校基金项目(2010XJZ24)

Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides

YAN Jun1，SUN Xiao-chun2，XIE Zhen-jian1，HE Gang1，YI Yong1，FANG Chen1，GOU Xiao-jun1,2,*

(1. Laboratory for Chemistry of Traditional Chinese Medicine, Chengdu University, Chengdu 610106, China； 2. Sichuan Industrial Institute of Antibiotic, Chengdu 610052, China)

Published:2011-10-12

摘要/Abstract

摘要： 采用水提醇沉法获得苦荞多糖，经离子交换柱分离，高效凝胶过滤色谱法(HPGFC)纯度鉴定并测定多糖的相对分子质量。硫酸水解后，用1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生水解后的单糖，衍生物采用反相高效液相色谱法测定单糖组成。结果表明：苦荞经水提醇沉，用Sevag 法脱蛋白后，获得苦荞多糖(TBP)；DEAE-纤维素柱层析获得3个苦荞多糖组分TBP-1、TBP-2和TBP-3，相对分子质量分别为144544、445656和636795。柱前衍生液相色谱分析可知：TBP-1、TBP-2是由葡萄糖组成的均一多糖；TBP-3由甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成的杂多糖，其物质的量比为4.32:2.41:1.00:39.8:9.64:2.02。

关键词: 苦荞, 多糖, 单糖, 衍生化, 高效液相色谱法

Abstract: Tartary buckwheat polysaccharides (TBPs) were extracted by boiling water, precipitated by alcohol and purified by ion-exchange column chromatography. The purity and molecular weight of TBPs were determined by high performance gel filtration chromatography (HPGFC). Polysaccharide samples were hydrolyzed with sulfuric acid solution, and derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition of the hydrolysates obtained was analyzed by RP-HPLC. The results showed that TBPs could be obtained by water extraction and alcohol precipitation after protein removal by Savag method. Three polysaccharides, named as TBP-1, TBP-2 and TBP-3, were obtained by DEAE-cellulose column chromatography with molecular weights of 144544, 445656 and 636795, respectively. Liquid chromatographic analysis based on pre-column derivatization showed that TBP-1 and TBP-2 were composed of glucose and TBP-3 of mannose, rhamnose, glucuronic acid, glucose, galactose and arabinose in a molar ratio of 4.32:2.41:1.00:39.8:9.64:2.02.

Key words: tartary buckwheat, polysaccharide, monosaccharide, derivatization, high performance liquid chromatography (HPLC)

中图分类号:

TS207.3

颜军，孙晓春，谢贞建，何钢，易勇，房晨，苟小军. 苦荞多糖的分离纯化及单糖组成测定[J]. 食品科学, 2011, 32(19): 33-36.

YAN Jun，SUN Xiao-chun，XIE Zhen-jian，HE Gang，YI Yong，FANG Chen，GOU Xiao-jun. Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides[J]. FOOD SCIENCE, 2011, 32(19): 33-36.

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苦荞多糖的分离纯化及单糖组成测定

Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides

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