一株串珠镰刀菌胞内红色素稳定性及部分生理活性研究

doi:10.7506/spkx1002-6630-201119012

食品科学 ›› 2011, Vol. 32 ›› Issue (19): 54-59.doi: 10.7506/spkx1002-6630-201119012

一株串珠镰刀菌胞内红色素稳定性及部分生理活性研究

孙月娥，邵颖，陈安徽，刘晓娟，樊美珍

1.徐州工程学院食品（生物）工程学院 2.安徽省微生物防治重点实验室

发布日期:2011-10-12

Stability and Biological Activity of Intracellular Red Pigment from Fusarium momiliforme

SUN Yue-e1，SHAO Ying1，CHEN An-hui1，LIU Xiao-juan2，FAN Mei-zhen2

(1. College of Food Science and Biotechnology, Xuzhou Institute of Technology, Xuzhou 221008, China； 2. Anhui Provincial Key Laboratory of Microbial Pest Control, Hefei 230036, China)

Published:2011-10-12

摘要/Abstract

摘要： 研究串珠镰刀菌RCEF4029菌株胞内红色素的稳定性及其部分生理活性。采用分光光度法测定红色素溶液的吸光度评价红色素的稳定性；DPPH自由基酶标仪法测定红色素溶液的抗氧化活性；中国仓鼠卵巢肿瘤细胞CHO Cytotoxicity Assay模型检测红色素溶液的抗肿瘤活性。结果显示：串珠镰刀菌RCEF4029菌株胞内红色素的热稳定性及光稳定性较好；常用金属离子中Zn2+、Ca2+对红色素稳定性基本无影响，Na+、Mg2+、Al3+具有增色效应，而Fe3+却破坏了色素的稳定性；氧化剂、还原剂、维生素、酸度调节剂、常用食品添加剂对胞内红色素稳定性影响较小，其中柠檬酸、氢氧化钠、VE、明胶和苯甲酸钠具有轻微增色作用。红色素对DPPH自由基具有一定的清除活性。

关键词: 串珠镰刀菌, 胞内红色素, 稳定性, 生理活性

Abstract: In order to explore the stability and biological activity of intracellular red pigment from Fusarium momiliforme RCEF4029, spectrophotometry was used to determine the stability of the red pigment and DPPH-microplate assay was used to evaluate its free radical scavenging activity. Meanwhile, a resazurin cytotoxicity assay model of Chinese hamster ovary (CHO) cells was employed to study anti-tumor activity of the pigment. The results showed that the red pigment had good thermal and light stability. Metal ions such as Zn2+ and Ca2+ had no obvious effect on the stability of the red pigment, while Na+, Mg2+ and Al3+ revealed hyperchromic effect on the red pigment; in contrast, Fe3+ had destructive effect on its stability. Oxidants, reductants, vitamins, acidity regulators and food additives had little effect on the stability of the red pigment. Citric acid, sodium hydrate, VE, gelatin and sodium benzoate had slight hyperchromic effect on its stability. Furthermore, the red pigment also exhibited some free radical scavenging activity. Therefore, the intracellular red pigment is a kind of stable natural pigment with wide utilization potential.

Key words: Fusarium momiliforme, intracellular red pigment, stability, biological activity

中图分类号:

TS202.3

孙月娥，邵颖，陈安徽，刘晓娟，樊美珍. 一株串珠镰刀菌胞内红色素稳定性及部分生理活性研究[J]. 食品科学, 2011, 32(19): 54-59.

SUN Yue-e，SHAO Ying，CHEN An-hui，LIU Xiao-juan，FAN Mei-zhen. Stability and Biological Activity of Intracellular Red Pigment from Fusarium momiliforme[J]. FOOD SCIENCE, 2011, 32(19): 54-59.

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一株串珠镰刀菌胞内红色素稳定性及部分生理活性研究

Stability and Biological Activity of Intracellular Red Pigment from Fusarium momiliforme

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