食品科学 ›› 2011, Vol. 32 ›› Issue (19): 217-219.doi: 10.7506/spkx1002-6630-201119048

• 营养卫生 • 上一篇    下一篇

沙棘叶水溶性多糖的抗突变作用

李芳亮,高 杨,刘 莹,杨新源,王 锐   

  1. 1.辽宁工程技术大学理学院 2.辽宁省阜新市科技局
  • 发布日期:2011-10-12
  • 基金资助:
    辽宁工程技术大学校优秀青年科学研究基金项目(07-127)

Anti-mutagenic Effect of Water-soluble Polysaccharide from Hippophae rhamnoides Leaves

LI Fang-liang1,GAO Yang2,LIU Ying1,YANG Xin-yuan1,WANG Rui1   

  1. (1. College of Science, Liaoning Technical University, Fuxin 123000, China; 2. Fuxin Science and Technology Bureau, Fuxin 123000, China)
  • Published:2011-10-12

摘要: 为了研究沙棘叶水溶性多糖(WPHL)的抗突变作用,本实验通过微核实验、精子畸变实验和彗星实验观察WPHL对环磷酰胺(CP)诱发的小鼠骨髓嗜多染红细胞微核、肝细胞DNA损伤和精子畸变的抑制作用。结果表明:WPHL 在50mg/(kg ·d)剂量时,对CP诱发的微核(P<0.05)和精子畸变(P<0.05)有明显的抑制作用,并可以显著减小肝脏彗星细胞的数量和缩短彗星细胞尾巴的长度(P<0.05)。说明有显著的抗突变能力。

关键词: 沙棘叶, 多糖, 抗突变, 微核实验, 彗星实验

Abstract: In order to explore the anti-mutagenic effect of water-soluble polysaccharides from Hippophae rhamnoides leaves (WPHL), the inhibitory effect of WPHL on bone mouse marrow cell micronuclei, abnormal sperm and hepatic DNA damage induced by cyclophosphamide (CP) was studied. The results show that WPHL at the dose of 50 mg/kg ·d significantly inhibited the frequency of micronucleus (P <0.05) and sperm abnormality (P <0.05). In addition, WPHL could also significantly decrease the number and tail length of hepatic comet cells (P <0.05). Therefore, WPHL has anti-mutagenic potential.

Key words: Hippophae rhamnoides leaf, polysaccharide, anti-mutation, micronuclei test, comet assay

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