食品科学 ›› 2011, Vol. 32 ›› Issue (19): 248-252.doi: 10.7506/spkx1002-6630-201119056

• 营养卫生 • 上一篇    下一篇

黑豆活性肽的分离纯化及其辅助降血脂作用

刘恩岐1,巫永华1,张建萍1,陈振家2,李玉娥2   

  1. 1.徐州工程学院 江苏省食品生物加工工程技术研究中心 2.山西农业大学食品科学与工程学院
  • 发布日期:2011-10-12
  • 基金资助:
    山西省回国留学人员科研资助项目(2007059)

Separation and Purification of Black Soybean Peptide and Its Hypolipidemic Effect in Mice

LIU En-qi1,WU Yong-hua1, ZHANG Jian-ping1, CHEN Zhen-jia2, LI Yu-e2   

  1. (1. Jiangsu Engineering Research Center for Food Biology Processing, Xuzhou Institute of Technology, Xuzhou 221008, China; 2. Food Science and Engineering College, Shanxi Agricultural University, Taigu 030801, China)
  • Published:2011-10-12

摘要: 采用DA201-C大孔树脂吸附和乙醇分级洗脱的方式,对黑豆蛋白酶解液进行分离纯化,通过体外模拟实验测定分析不同体积分数乙醇洗脱的黑豆肽分级组分对胆固醇胶束溶解度的抑制率,选择对胆固醇胶束溶解度抑制率最强的黑豆肽组分进行辅助降血脂动物实验。结果表明:黑豆肽分级组分的平均疏水性值随着洗脱乙醇体积分数的增大而递增,其中乙醇体积分数为75%时洗脱得到的组分对胆固醇胶束溶解度的抑制率最高,该组分添加量为5g/L时,其抑制率为62.1%;对小鼠灌胃剂量为1000mg/(kg ·d)时,黑豆肽对高血脂小鼠血清中总胆固醇(TC)、总甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)+极低密度脂蛋白胆固醇(VLDL-C)浓度降低极为显著(P<0.01)。提示黑豆肽具有一定的辅助降血脂功效。

关键词: 黑豆肽, 大孔吸附树脂, 分离纯化, 胆固醇胶束溶解度, 降血脂

Abstract: Macroporous adsorption resin DA201-C was used for the isolation of hypolipidemic peptides from black soybean protein hydrolysate. The inhibitory rates of cholesterol micelle solubility (CMSIR) by all fractions were determined, and the hypolipidemic effect of the fraction with the highest CMSIR was evaluated in mice. The results showed that black soybean peptides were eluted from DA201-C resin according to its hydrophobicity through gradient ethanol elution. The peptide fraction eluted by 75% ethanol displayed the highest CMSIR of 62.1% when the concentration of peptide fraction was 5 g/L. After administration of the peptide at the dose of 1000 mg/(kg ·d) for 30 days in mice, the levels of serum TC, TG and LDL-C and VLDL-C were obviously decreased (P <0.01) when compared with the mice fed hypercholesterolemia diet.

Key words: black soybean peptides, macroporous resin, separation and purification, cholesterol micelle solubility, hypolipidemic activity

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