食品科学 ›› 2011, Vol. 32 ›› Issue (20): 84-88.doi: 10.7506/spkx1002-6630-201120018

• 工艺技术 • 上一篇    下一篇

响应曲面法优化木瓜蛋白酶改善蛋清蛋白起泡性能工艺

涂勇刚1,聂旭亮2,徐明生1,孙春妹1,杜华英1,赵 燕3,董 攀3   

  1. 1.江西农业大学食品科学与工程学院 2.江西农业大学理学院 3.南昌大学生物质转化教育部工程研究中心
  • 出版日期:2011-10-25 发布日期:2011-10-12
  • 基金资助:
    江西省教育厅青年科学基金项目(GJJ11088);江西农业大学自然科学青年基金项目(9003315); 江西省科技厅科技支撑计划项目(20112BBF60016)

Response Surface Methodology for Technological Optimization of Papain Hydrolysis of Albumen Protein for Improved Foaming Properties

TU Yong-gang1,NIE Xu-liang2,XU Ming-sheng1,SUN Chun-mei1,DU Hua-ying1,ZHAO Yan3,DONG Pan3   

  1. (1. College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China; 2. College of Science, Jiangxi Agricultural University, Nanchang 330045, China; 3. Engineering Research Center of Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China)
  • Online:2011-10-25 Published:2011-10-12

摘要: 优化木瓜蛋白酶改善蛋清蛋白起泡性能的工艺。在单因素试验基础上,确定自变量酶解时间、木瓜蛋白酶添加量、酶解温度、pH值的试验水平,以起泡性和泡沫稳定性为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对蛋清蛋白起泡性和泡沫稳定性的影响。然后利用Design Expert软件进行响应面分析,模拟得到二次多项式回归方程的预测模型,并确定蛋清蛋白酶改性的最佳条件组合。结果表明:蛋清蛋白泡沫稳定性酶解模型不显著,而起泡性酶解模型显著,在最佳工艺参数酶解时间45min、酶添加量38.2mg/5mL蛋清、酶解温度47.5℃、pH6.3的条件下,蛋白起泡性可达2.09,此条件下可使蛋清蛋白起泡性提高140%左右。

关键词: 蛋清蛋白, 木瓜蛋白酶, 起泡性, 泡沫稳定性, 响应曲面法

Abstract: The purpose of this study is to optimize the improvement of foaming properties of albumen protein by papain hydrolysis. Foaming capacity and foam stability were investigated with respect to 4 variables including hydrolysis time, papain dosage, temperature and pH. Two quadratic polynomial regression models describing foaming capacity and foam stability were established based on a Box-Behnken experimental design. According to response surface analysis, the foam stability model was not statistically significant, the foaming capacity model displayed a statistical significance, and the optimum enzymatic modification conditions were hydrolysis time of 45 min, enzyme dosage of 38.2 mg/5 mL of egg white, hydrolysis temperature of 47.5℃, and pH 6.3. Under the optimum conditions, the foaming capacity was up to 2.09, indicating an increase of approximately 140% when compared to non-modified albumen protein.

Key words: albumen protein, papain, foaming ability, foam stability, response surface methodology

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