食品科学 ›› 2012, Vol. 33 ›› Issue (6): 92-95.doi: 10.7506/spkx1002-6630-201206020

• 工艺技术 • 上一篇    下一篇

响应面法优化啤酒酵母泥中β-1,3-葡聚糖提取工艺

张桂香,王元秀*,宋晓妍,孙纳新,李 戎   

  1. 济南大学医学与生命科学学院
  • 出版日期:2012-03-25 发布日期:2012-03-03
  • 基金资助:
    山东省教育厅科技立项项目(J09LC21);济南大学科研基金项目(XKY0928)

Optimization of Extraction Process for β-1,3-Glucan from Yeast Paste by Response Surface Methodology

ZHANG Gui-xiang,WANG Yuan-xiu*,SONG Xiao-yan,SUN Na-xin,LI Rong   

  1. (College of Medicine and Life Sciences, University of Jinan, Jinan 250022, China)
  • Online:2012-03-25 Published:2012-03-03

摘要: 为提高啤酒酵母泥中β-1,3-葡聚糖的得率,采用响应面法优化酸碱法提取葡聚糖的工艺条件,对NaOH质量分数、碱提温度、碱提时间3个因素进行单因素试验。根据单因素试验结果,设计中心组合试验,以葡聚糖得率为指标,采用响应面分析法确定最优工艺参数。结果表明:在NaOH溶液质量分数2.05%、92.70℃处理2.50h条件下,β-1,3-葡聚糖得率实测值为15.76%,模型预期值为15.82%,响应面优化法提高了β-1,3-葡聚糖的得率。

关键词: β-1, 3-葡聚糖, 啤酒酵母泥, 响应面法, 提取工艺

Abstract: In order to improve the extraction rate of β-1,3-glucan from yeast paste, response surface methodology was used for the optimization of alkaline extraction conditions. The effects of NaOH concentration, extraction time and extraction temperature on the extraction rate of β-1,3-glucan were explored by single factor tests. Subsequently, a regression model was established using a central composite design. Based on the model, response surface analysis was conducted to explore optimal extraction conditions. The results indicated that the optimal extraction process parameters were NaOH concentration of 2.05%, extraction temperature of 92.70 ℃ and extraction time of 2.50 h. Under these optimal extraction conditions, the extraction rate of β-1,3-glucan was up to 15.76%, which was consistent with the predicted value of 15.82%. Therefore, the extraction rate of β-1,3-glucan were enhanced by response surface methodology.

Key words: β-1,3-glucan, beer yeast paste, response surface methodology (RSM), extraction process

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