食品科学 ›› 2012, Vol. 33 ›› Issue (6): 96-99.doi: 10.7506/spkx1002-6630-201206021

• 工艺技术 • 上一篇    下一篇

普通念珠藻多糖提取及其体外抑菌活性研究

钱森和1,厉荣玉2,魏 明1,杨超英1   

  1. 1.安徽工程大学生物与化学工程学院 2.皖南医学院
  • 出版日期:2012-03-25 发布日期:2012-03-03
  • 基金资助:
    安徽省高校省级科学研究项目(KJ2010B285;KJ2011B201)

Extraction and Antibacterial Activity of Polysaccharides from Nostoc commune

QIAN Sen-he1,LI Rong-yu2,WEI Ming1,YANG Chao-ying1   

  1. (1. College of Biology and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China; 2. Wannan Medical College, Wuhu 241001, China)
  • Online:2012-03-25 Published:2012-03-03

摘要: 通过单因素和正交试验对普通念珠藻多糖的提取条件进行优化,并采用滤纸扩散法对普通念珠藻多糖的抑菌作用进行研究。结果表明:提取温度和提取时间对普通念珠藻多糖得率的影响分别达到了极显著和显著水平,最佳提取条件为提取温度90℃、提取时间3h、料液比1:15(g/mL)、提取2次;普通念珠藻多糖对大肠杆菌、枯草芽孢杆菌、粘质沙雷细菌、黑曲霉和假丝酵母具有较好的抑制作用,其对大肠杆菌、枯草芽孢杆菌和粘质沙雷氏菌的最小抑菌质量浓度分别为50、50、25mg/mL,对黑曲霉和假丝酵母的最低抑制质量浓度为100mg/mL。

关键词: 普通念珠藻, 多糖, 提取, 抑菌

Abstract: This paper deals with the optimization of process conditions for the extraction of polysaccharides from Nostoc commune using one-factor-at-a-time and orthogonal array design methods. Meanwhile, the antibacterial activity of polysaccharides from Nostoc commune was tested by the filter paper disc diffusion method. The results showed that extraction temperature and time influenced the extraction efficiency of polysaccharides from Nostoc commune at an extremely significant level and at a significant level, respectively. The optimal extraction conditions were temperature of 90 ℃, extraction time of 3 h, material-liquid ratio of 1:15, and repeated extraction number of 2. The resulting extract revealed good antibacterial activity against Escherichia coli, Bacillus subtilis, Serratia marcescens, Aspergillus nigere and Candida albicans with minimum inhibitory concentration (MIC) of respectively 50, 50, 25, 100 mg/mL and 100 mg/mL.

Key words: Nostoc commune, polysaccharide, extract, antibacterial

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