食品科学 ›› 2012, Vol. 33 ›› Issue (6): 154-158.doi: 10.7506/spkx1002-6630-201206033

• 分析检测 • 上一篇    下一篇

鸡蛋中磺胺类残留物提取方法

钟子清1,黄卓焕2,赖卫华1,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2.江西省农产品质量安全检测中心
  • 出版日期:2012-03-25 发布日期:2012-03-03

A Comparative Study on LC-MS/MS Determination of Sulfonamide Residues in Hen,s Eggs Based on Different Sample Pretreatments

 ZHONG Zi-qing1,HUANG Zhuo-huan2,LAI Wei-hua1,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;   2. Jiangxi Agricultural Product Quality Safety and Inspection Center, Nanchang 330077, China)
  • Online:2012-03-25 Published:2012-03-03

摘要: 为获得鸡蛋中磺胺类药物残留检测的提取方法,分别在鸡蛋中添加高、中、低浓度的磺胺类药物,根据GB/T 20759-2006《畜禽肉中十六种磺胺类药物残留量的测定:液相色谱-串联质谱法》的方法以及自行设计方法对样品进行前处理,用液相色谱-串联质谱检测。结果表明:添加量在10~200ng/g范围内,鸡蛋中回收率为20.8%~69.29%,回收率未能达到标准;GB/T 20759-2006在提取、浓缩、除脂、标准曲线绘制方面对于禽蛋类食品不适用,需根据禽蛋类的特殊性制定专门的检测标准,而自行设计方法所得回收率为77.92%~95.26%,可以满足检测的要求。

关键词: 磺胺类药物, 鸡蛋, 前处理

Abstract: Two different sample pretreatment methods, namely the national standard method GB/T 20759-2006 and a method developed in our laboratory were compared for their application in LC-MS/MS determination of sulfonamide residues in hen,s eggs. The average recoveries of the national standard method across the spike levels of 10, 100 ng/g and 200 ng/g ranged from 20.8%-67.7%, which could not meet the requirements. Moreover, the method was not suitable for use in the extraction, concentration, defatting, and standard curve preparation of egg foods. Therefore, the determination of sulfonamide residues in eggs requires a new specialized method due to their special characteristics. The average recoveries of the self-developed method were between 77.92% and 95.26% and could meet the requirements for the determination of sulfonamide residues.

Key words: sulfonamide, hen,s egg, sample pretreatment

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