食品科学 ›› 2013, Vol. 34 ›› Issue (5): 207-212.doi: 10.7506/spkx1002-6630-201305043

• 生物工程 • 上一篇    下一篇

响应面法优化长双歧杆菌增殖培养基

孙 鹏1,赵旭博2,孙先锋1,马永征3   

  1. 1.西安工程大学环境与化学工程学院,陕西 西安 710048;2.西北农林科技大学食品科学与工程学院, 陕西 杨凌 712100;3.北京市食品研究所,北京 100162
  • 收稿日期:2012-09-16 修回日期:2013-01-12 出版日期:2013-03-15 发布日期:2013-04-16
  • 通讯作者: 孙鹏 E-mail:sunpengtg@gmail.com

Optimization of Enrichment Medium for Bifidobacterium longum by Response Surface Methodology

SUN Peng 1,ZHAO Xu-bo2,SUN Xian-feng1,MA Yong-zheng3   

  1. 1. School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710048, China; 2. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; 3. Beijing Food Research Institute, Beijing 100162, China
  • Received:2012-09-16 Revised:2013-01-12 Online:2013-03-15 Published:2013-04-16
  • Contact: SUN Peng E-mail:sunpengtg@gmail.com

摘要: 为了提高长双歧杆菌发酵液中的活菌数,对其增殖培养基进行响应面优化。通过单因素试验筛选出长双歧杆菌的最佳碳源为乳糖,并发现低聚木糖、菊糖、低聚异麦芽糖、低聚果糖、苯丙氨酸、蛋氨酸、脯氨酸、谷氨酸及赖氨酸均能显著促进长双歧杆菌的生长。利用Design Expert 8.06软件设计Plackett-Burman 试验筛选出影响长双歧杆菌生长的3个最重要因子,通过Box-Behnken试验及响应面分析确定3个因子的最佳添加量为:低聚木糖1.7g/L、菊糖3.6g/L、脯氨酸0.4g/L,用优化后的增殖培养基培养长双歧杆菌,18h后其活菌数达(1.75±0.02)×109CFU/mL,比优化前提高了95.64%。

关键词: 长双歧杆菌, 增殖培养基, 响应面法, 益生元, 氨基酸

Abstract: In order to improve viable Bifidobacterium longum, the optimization of enrichment medium was carried out by response surface methodology. The optimal carbon source for Bifidobacterium longum was lactose. Xylooligosaccharide, inulin, isomaltooligosaccharide, fructooligosaccharide, phenylalanine, methionine, proline, glutamic acid and lysine were found to significantly promote the growth of Bifidobacterium longum. Three most important nutrients for Bifidobacterium longum were screened via Plackett-Burman using software of Design expert 8.06. Optimal concentrations of three substrates were confirmed by Box-Behnken and response surface analysis to be 1.7 g/L of xylooligosaccharide, 3.6 g/L of inulin and 0.4 g/L proline. Cultivated in optimal enrichment medium, the viable counts of Bifidobacterium longum after 18 h reached up to (1.75 ± 0.02)×109 CFU/mL, which revealed an increase by 95.64% when compared with the original medium.

Key words: Bifidobacterium longum, enrichment medium, response surface methodology, prebiotics, amino acid

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