食品科学 ›› 2013, Vol. 34 ›› Issue (8): 164-167.doi: 10.7506/spkx1002-6630-201308034

• 分析检测 • 上一篇    下一篇

UPLC法测定大豆制品及相关制剂中大豆异黄酮含量

袁波,甄慧娟,姜璇,孙帅婷,许丽,徐海燕   

  1. 沈阳药科大学药学院,辽宁 沈阳 110016
  • 收稿日期:2011-12-11 修回日期:2013-03-13 出版日期:2013-04-25 发布日期:2013-05-07
  • 通讯作者: 徐海燕 E-mail:xhy1020@gmail.com
  • 基金资助:
    国家自然科学基金资助项目

Determination of Isoflavones in Soybean Products and Supplements by UPLC

YUAN Bo,ZHEN Hui-juan,JIANG Xuan,SUN Shuai-ting,XU Li,XU Hai-yan   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2011-12-11 Revised:2013-03-13 Online:2013-04-25 Published:2013-05-07

摘要: 目的:建立同时测定大豆制品及相关制剂中4种大豆异黄酮类化合物大豆苷、染料木苷、大豆苷元、染料木素含量的超高效液相色谱法。方法:色谱柱:Acquity UPLC BEH C18柱(50mm×2.1mm,1.7μm),流动相:A为体积分数0.2%甲酸溶液,B为乙腈,梯度洗脱;流速:0.4mL/min;检测波长:254nm,柱温:30℃。结果:线性范围:大豆苷0.625~40.0mg/L(r=1.000),染料木苷0.625~40.0mg/L(r=0.9999),大豆苷元0.04~2.56mg/L(r=0.9997),染料木素0.04~2.56mg/L(r=0.9996)。大豆和Natrol? soy isoflavones中大豆苷、染料木苷、大豆苷元、染料木素平均回收率为98.5%~99.8%。结论:本方法简便、快速、灵敏、准确,适用于大豆制品及相关制剂中大豆异黄酮含量测定。

关键词: 超高效液相色谱法, 大豆制品, 大豆异黄酮, 含量测定

Abstract: Objective: To develop an ultra-high performance liquid chromatography (UPLC) method for the determination of four major isoflavones such as daidzin, genistin, daidzein and genistein in soybean products and supplements. Methods: The analytes were separated on an Acquity UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm) with a mobile phase consisting of 0.2% formic acid and acetonitrile in gradient mode, and UV detection at 254 nm. The flow rate was 0.4 mL/min. The column temperature was 30 ℃. Results: A good linear relationship within the range of 0.625 to 40.0 mg/L for daidzin (r = 1.000), 0.625 to 40.0 mg/L for genistin (r = 0.9999), 0.04 to 2.56 mg/L for daidzein (r = 0.9997) and 0.04 to 2.56 mg/L for genistein (r = 0.9996) was observed. The average recovery rates of daidzin, genistin, daidzein and genistein were 98.5%–99.8%. Conclusion: The developed method is fast, simple, sensitive, and accurate for the determination of four isoflavones in soybean products and supplements.

Key words: ultra-high performance liquid chromatography (UPLC), soybean products, isoflavone, quantitative determination

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