富硒玉米蛋白水解物中硒肽及含硒氨基酸的结构鉴定

doi:10.7506/spkx1002-6630-201309001

食品科学 ›› 2013, Vol. 34 ›› Issue (9): 1-4.doi: 10.7506/spkx1002-6630-201309001

• 基础研究 • 下一篇

富硒玉米蛋白水解物中硒肽及含硒氨基酸的结构鉴定

王真真，张久亮，王驰，侯焘，张岩，何慧*

华中农业大学食品科学技术学院，环境食品学教育部重点实验室，湖北武汉 430070

收稿日期:2013-03-20 修回日期:2013-04-11 出版日期:2013-05-15 发布日期:2013-05-07
通讯作者: 何慧 E-mail:hehui@mail.hzau.edu.cn
基金资助:
国家自然科学基金项目(30972043)；国家“863”计划项目(2008AA10Z314)

Structural Identification of Seleno-peptides and Seleno-amino Acids in Se-enriched Corn Protein Hydrolysates

WANG Zhen-zhen，ZHANG Jiu-liang，WANG Chi，HOU Tao，ZHANG Yan，HE Hui*

Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology,
Huazhong Agricultural University, Wuhan 430070, China

Received:2013-03-20 Revised:2013-04-11 Online:2013-05-15 Published:2013-05-07
Contact: HE Hui E-mail:hehui@mail.hzau.edu.cn

摘要/Abstract

摘要：

为了明确硒在玉米蛋白中的赋存形式，采用高效液相色谱-质谱联用法(HPLC-MS/MS)，结合质谱数据库比对以及高效液相色谱-等离子体质谱法(HPLC-ICP/MS)，对富硒玉米蛋白水解物中硒肽及含硒氨基酸的结构进行鉴定。结果确定了5个小肽，其氨基酸序列分别为：SeMet-MeSeCys-Glu、Met-MeSeCys-Glu、MeSeCys-Glu-Asp、Ile-MeSeCys-Glu、γ-Glu-MeSeCys；确定了4种含硒氨基酸，分别为硒代蛋氨酸(SeMet)、硒代乙硫氨酸(SeEt)、L-硒-甲基硒代半胱氨酸(MeSeCys)、硒代胱氨酸(SeCys2)。借助于标样对含硒氨基酸和二肽进行定量，5个硒肽均含MeSeCys，其中只有一个肽段含SeCys2；但在含硒氨基酸中则是以SeMet含量最高为(586.3±49.5)ng/g，最低的是SeCys2为(102.6±9.0)ng/g。

关键词: 富硒玉米, 硒肽, 含硒氨基酸, 液质联用法, 高效液相色谱-等离子体质谱法

Abstract:

In order to clarify the combination form of selenium in corn proteins, the structures of seleno-peptides in Se-enriched
corn protein hydrolysates were identified by HPLC-MS/MS method coupled with mass spectrum database and seleno-amino
acids were identified by HPLC-ICP-MS method. Five seleno-peptides were confirmed as SeMet-MeSeCys-Glu, Met-MeSeCys-
Glu, MeSeCys-Glu-Asp, Ile-MeSeCys-Glu and γ-Glu-MeSeCys, and four seleno-amino acids were identified as selenomethionine
(SeMet), eleno-Ethionine (SeEt), Se-methylseleno-L-Cysteine (MeSeCys) and selenocystine (SeCys2), respectively. Seleno-amino
acids and dipeptide were been quantified using standard samples. Selenomethionine (SeMet) revealed the highest content (586.3 ±
49.5) ng/g, and Selenocystine (SeCys2) revealed the low content (102.6 ± 9.0) ng/g. Based on the above results, all five selenopeptides
contain MeSeCys, and only one contains SeMet. The major seleno-amino acid was SeMet.

Key words: Se-enriched corn, seleno-peptides, seleno-amino acids, HPLC-MS/MS, HPLC-ICP/MS

中图分类号:

王真真，张久亮，王驰，侯焘，张岩，何慧*. 富硒玉米蛋白水解物中硒肽及含硒氨基酸的结构鉴定[J]. 食品科学, 2013, 34(9): 1-4.

WANG Zhen-zhen，ZHANG Jiu-liang，WANG Chi，HOU Tao，ZHANG Yan，HE Hui*. Structural Identification of Seleno-peptides and Seleno-amino Acids in Se-enriched Corn Protein Hydrolysates[J]. FOOD SCIENCE, 2013, 34(9): 1-4.

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富硒玉米蛋白水解物中硒肽及含硒氨基酸的结构鉴定

Structural Identification of Seleno-peptides and Seleno-amino Acids in Se-enriched Corn Protein Hydrolysates

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