不同品种花生蛋白主要组分及其亚基相对含量分析

doi:10.7506/spkx1002-6630-201309010

食品科学 ›› 2013, Vol. 34 ›› Issue (9): 42-46.doi: 10.7506/spkx1002-6630-201309010

不同品种花生蛋白主要组分及其亚基相对含量分析

杜寅，王强*，刘红芝，王丽，刘丽

中国农业科学院农产品加工研究所，农业部农产品加工综合性重点实验室，北京 100193

收稿日期:2012-05-18 修回日期:2013-03-26 出版日期:2013-05-15 发布日期:2013-05-07
通讯作者: 王强 E-mail:wangqiang365@263.net
基金资助:
国家公益性行业(农业)科研专项(200903043)；中央级公益性科研院所基本科研业务费专项(zwjk201201)

Major Protein Fractions and Subunit Contents in Peanut from Different Cultivars

DU Yin，WANG Qiang*，LIU Hong-zhi，WANG Li，LIU Li

Key Laboratory of Agro-products Processing, Ministry of Agriculture, Institute of Agro-products Processing Science and Technology,
Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2012-05-18 Revised:2013-03-26 Online:2013-05-15 Published:2013-05-07
Contact: Qiang Wang E-mail:wangqiang365@263.net

摘要/Abstract

摘要：

对我国主要花生种植区的170个花生品种的蛋白质组成进行SDS-PAGE电泳分析。结果表明：不同花生品种的蛋白组分和亚基相对含量在种质材料间存在差异。各品种均含有花生球蛋白和伴花生球蛋白，花生球蛋白与伴花生球蛋白比值变化范围介于0.80～1.68之间，变异系数为15.23%，大于10%，说明不同花生品种蛋白组成比例存在较大的变异性。各品种亚基变异显著，其中，花生球蛋白的35.5kD亚基差异最显著，变异系数达55.07%，双纪2号等35个品种缺失35.5kD亚基条带，占所测试品种的20.59%。相关性分析表明，花生球蛋白与伴花生球蛋白之间呈极显著负相关(r=－0.998)。

关键词: 花生蛋白, 花生球蛋白, 伴花生球蛋白, 亚基组成, 相对含量

Abstract:

The composition of peanut protein from 170 different cultivars in China was analyzed by SDS-PAGE as well
as the relative content of major fractions. There were significant variations in protein composition and the relative content
of subunits among these peanut cultivars. Both arachin and conarachin were found in each cultivar, with a ratio between
0.80 and 1.68, and a coefficient of variation (CV) of 15.23%. This CV larger than 10% suggests great differences in protein
composition among different peanut cultivars. In addition, significant variations were also observed in the relative content
of subunits; in arachin, the 35.5 kD subunit presented the largest CV of 55.07%, which, however, was absent from 20.59%
(35/170, such as ShuangJi 35) of all the tested cultivars. Moreover there was an extremely significantly negative correlation
between arachin and conarachin(r = －0.998).

Key words: peanut protein, arachin, conarachin, subunit composition, relative content

中图分类号:

TS201.2

杜寅，王强*，刘红芝，王丽，刘丽. 不同品种花生蛋白主要组分及其亚基相对含量分析[J]. 食品科学, 2013, 34(9): 42-46.

DU Yin，WANG Qiang*，LIU Hong-zhi，WANG Li，LIU Li. Major Protein Fractions and Subunit Contents in Peanut from Different Cultivars[J]. FOOD SCIENCE, 2013, 34(9): 42-46.

参考文献

[1] 高焕春, 李文英, 吕晓玲. 花生蛋白质稳定性及加工品工艺条件的研究[J]. 中外技术情报, 1995, 12: 39-40.
[2] 黎茵, 黄上志, 傅家瑞. 不同品种花生种子蛋白质的电泳分析[J]. 植物学报, 1998, 40(6): 534-541.
[3] Yamada T, Aibara S, Morita Y. Isolation and some properties of arachin subunits[J]. Agricultural and Biological Chemistry, 1979, 43: 2563-2568.
[4] Krishna T G, Pawar S E, Mitra R. Variation and inheritance of the arachin polypeptides of groundnut (Arachis hypogaea L.).Theor Appl Genet,1986,73:82-87.
[5] 高云中. 花生粕蛋白的提取及性质研究[硕士学位论文]. 江苏: 江南大学, 2009, p6.
[6] 王延华, 邹晓莉主编. 蛋白质理论与技术. 北京: 科学出版社, 2004.
[7] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680-685.
[8] 宋鹏, 周瑞宝, 魏安池. 大豆蛋白亚基组成对其功能特性影响的研究现状[J]. 粮油食品科技, 2010, 18(5): 19-22.
[9] Saio, K. Food processing characteristics of soybean 11S and 7S protein Part Ⅰ. Effect of difference of protein component among soybean varieties on formation of tofu-curd [J]. Agric Biol Chem., 1969, (33): 1301-1308.
[10] Arrese E L, Sorgentini D A, Wagner J R, et al. Electrophoretic, solubility, and functional properties of commercial soy protein isolates[J]. J. Agric. Food Chem. 1991, 39(6): 1033-1036.
[11] Mujoo R, Trinh D T, Ng P K W. Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J]. Food Chemistry, 2003, 82: 265-273.
[12] 程翠林, 王振宇, 石彦国等. 大豆蛋白亚基组成与7S/11S对豆腐品质及产率的影响[J]. 中国油脂, 2006, 31(4): 16-19.
[13] Shokarii E H, Esen A, Mozingo R W. Immunological characterization of a 36 kD polypeptide in peanuts (Arachis hypogaea L.)[J]. Peanut Sci, 1991, 18: 11-15.
[14] 杨晓泉, 陈中, 赵谋明. 花生蛋白的分离及部分性质研究[J]. 中国粮油学报, 2001, 16(5): 25-28.
[15] Utsumi S, Kinsella J E. Structure function relationships in food proteins: subunit interactions in heat-induced gelation of 7S,11S, and soy isolate proteins [J]. Agric. Food Chem., 1985, 33(2): 297-303.
[16] Utsumi S, Kinsella J E. Forces involved in soy protein gelation: effects of various reagents on the formation, hardness and solubility of heat-induced gels made from 7S,11S and soy isolate. [J]. J. Food Sci, 1985, (50): 1278-1282.
[17] S.L.TAY. Physicochemical properties of 7S and 11S protein mixtures coagulated by Glucono-δ-lactone[J]. J. Food Sci, 2004, 69(4): 139-143.
[18] 刘春, 王显生, 麻浩. 大豆种子贮藏蛋白亚基特异种质的蛋白功能性评价[J]. 中国油脂, 2008, 33(8): 31-36.
[19] Salleh, Nobuyuki M, Koji T, et al. Gelling properties of soybean β-conglycinin having different subunit compositions [J]. Biochem, 2004, 68(5): 1091-1096.

不同品种花生蛋白主要组分及其亚基相对含量分析

Major Protein Fractions and Subunit Contents in Peanut from Different Cultivars

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