食品科学 ›› 2013, Vol. 34 ›› Issue (9): 99-103.doi: 10.7506/spkx1002-6630-201309022

• 生物工程 • 上一篇    下一篇

酵母原生质体制备、融合及高产β-胡萝卜素融合子的筛选

王岁楼,王海翔,杨志萍,吕春晖,步 芬   

  1. 中国药科大学药学院,江苏 南京 210009
  • 收稿日期:2012-06-12 修回日期:2013-04-10 出版日期:2013-05-15 发布日期:2013-05-07
  • 通讯作者: 王岁楼 E-mail:cpuwsl@126.com
  • 基金资助:

    国家自然科学基金项目

Preparation and Fusion of Yeast Protoplasts and Screening of Fusants with High Yield of β-Carotene

WANG Sui-lou,WANG Hai-xiang,YANG Zhi-ping,LÜ Chun-hui,BU Fen   

  1. College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
  • Received:2012-06-12 Revised:2013-04-10 Online:2013-05-15 Published:2013-05-07
  • Contact: Sui-Lou WANG E-mail:cpuwsl@126.com

摘要:

为提高黏红酵母NR-98(生长温度28℃较高,但β-胡萝卜素产量较低)β-胡萝卜素发酵的产率,将其与法夫酵母Ph-1(β-胡萝卜素产量较高,但生长温度只有20℃)进行了原生质体融合。通过研究两种酵母菌原生质体制备、再生及融合的影响因素,最终筛选出了既能在28℃较高温度下生长、β-胡萝卜素产量又高于两出发菌株的具有遗传稳定性的融合子R-5,其β-胡萝卜素产量不仅比黏红酵母NR-98提高了53.33%,而且比法夫酵母Ph-1也提高了27.42%,达到9.20mg/L。

关键词: 酵母;原生质体融合;&beta, -胡萝卜素

Abstract:

The protoplast fusion technique between R. glutinis NR-98 and Phaffia rhodozyma Ph-1 was studied. The strain
NR-98 could grow at 28 ℃, but had a lower yield of β-carotene. In contrast, the strain Ph-1 had high yield of β-carotene, but
could not grow at 28 ℃. The results of fusion experiments showed that the fusant R-5 revealed the parent strain properties
of thermotolerance and β-carotene yield. When using glucose as substrate at 28 ℃, the fusant R-5 had a high β-carotene
yield up to 9.20 mg/L, with the enhancement of 27.42% and 53.33% when compared with strain Ph-1 and strain NR-98,
respectively. The genetic quality of fusant R-5 was good as confirmed by stability experiments.

Key words: yeasts;protoplast fusion;&beta, -carotene

中图分类号: