酪蛋白糖巨肽和乳铁蛋白灌胃对小鼠肠道微生物定殖抗力的影响

doi:10.7506/spkx1002-6630-201309057

食品科学 ›› 2013, Vol. 34 ›› Issue (9): 282-286.doi: 10.7506/spkx1002-6630-201309057

酪蛋白糖巨肽和乳铁蛋白灌胃对小鼠肠道微生物定殖抗力的影响

任效东，陈庆森，李俊洁，周丽丽，阎亚丽

天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300134

收稿日期:2013-01-23 修回日期:2013-04-15 出版日期:2013-05-15 发布日期:2013-05-07
基金资助:
国家自然科学基金资助项目

Effect of Administation of CGMP and Lactoferrin by Gavage on Colonization Resistance in the Intestine of Mice

REN Xiao-dong，CHEN Qing-sen*，LI Jun-jie，ZHOU Li-li，YAN Ya-li

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134, China

Received:2013-01-23 Revised:2013-04-15 Online:2013-05-15 Published:2013-05-07

摘要/Abstract

摘要： 研究探讨酪蛋白糖巨肽(casein glycomacropeptide，CGMP)和乳铁蛋白(lactoferrin，Lf)对小鼠肠道双歧杆菌属数量与肠杆菌属数量对数值比值(B/E值)的影响。选取48只BALB/c小鼠随机分成8组：对照组(日食组)，安慰组(灌胃生理盐水0.2mL)，低、中、高剂量CGMP组(灌胃等体积不同质量浓度的CGMP溶液)，低、中、高剂量Lf组(灌胃等体积不同质量浓度的Lf溶液)，灌胃周期为14d。分别在灌胃前以及实验的第3、7、11、15天和停止灌胃后6d(第21天)用逼迫法采集小鼠新鲜粪便，采用选择性培养基进行肠道中双歧杆菌与肠杆菌数量的测定。结果表明：中剂量的CGMP(0.5mg/mL)和Lf(0.1mg/mL)均可促进双歧杆菌增殖且抑制肠杆菌增殖，B/E值增加，且CGMP促进小鼠肠道B/E值的增长最为显著。研究证实CGMP和乳铁蛋白在一定程度上可以使肠道B/E值增加，并且对小鼠肠道菌群具有一定的调节作用。

关键词: CGMP, 乳铁蛋白, 灌胃, 肠道B/E值

Abstract: This study explored the effect of casein glycomacropeptide (CGMP) and lactoferrin (Lf) on the logarithmic ratio of Bifidobacterium to Enterobacter (B/E) in the intestine of mice. BALB/c mice were randomly divided into eight groups: control (daily diet), placebo (0.2 mL of normal saline), low-, middle-, and high-dose CGMP treatments ( the same volume as normal saline at different concentrations), and low-, middle-, and high-dose Lf treatments (the same volume as normal saline at different concentrations). The administration period was 14 d. Fresh feces were collected from all mice before administration, on the 3rd, 7th, 11th and 15th days of administation and 6 d after the end of the administration period for enumeration of Bifidobacterium and Enterobacter after culture in selective media. Both CGMP and Lf at the middel dose stimulated the growth of Bifidobacterium but exerted a growth-inhibiting effect on Enterobacter, leading to an increase in B/E ratio, which was more obviously increased by CGMP. This study has demonstrated that both CGMP and Lf can increase the intestinal B/E ratio of mice and modulate the intestinal microflora.

Key words: CGMP, lactoferrin, gavage, vintestinal B/E ratio

中图分类号:

TS252

任效东，陈庆森，李俊洁，周丽丽，阎亚丽. 酪蛋白糖巨肽和乳铁蛋白灌胃对小鼠肠道微生物定殖抗力的影响[J]. 食品科学, 2013, 34(9): 282-286.

REN Xiao-dong，CHEN Qing-sen*，LI Jun-jie，ZHOU Li-li，YAN Ya-li. Effect of Administation of CGMP and Lactoferrin by Gavage on Colonization Resistance in the Intestine of Mice[J]. FOOD SCIENCE, 2013, 34(9): 282-286.

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酪蛋白糖巨肽和乳铁蛋白灌胃对小鼠肠道微生物定殖抗力的影响

Effect of Administation of CGMP and Lactoferrin by Gavage on Colonization Resistance in the Intestine of Mice

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