1,3-二氯-2-丙醇对睾丸间质细胞R2C活性及孕酮合成的影响

doi:10.7506/spkx1002-6630-201309059

食品科学 ›› 2013, Vol. 34 ›› Issue (9): 292-295.doi: 10.7506/spkx1002-6630-201309059

1,3-二氯-2-丙醇对睾丸间质细胞R2C活性及孕酮合成的影响

白顺1，孙建霞2，白卫滨3,*，邹飞雁1，张齐好4，黄亚东4

1.暨南大学发育与再生生物学系，广东广州 510632；2.广东工业大学轻工化工学院，广东广州 510006；
3.暨南大学食品科学与工程系，广东广州 510632；4.暨南大学医药生物技术研究开发中心，广东广州 510632

收稿日期:2012-06-20 修回日期:2013-04-11 出版日期:2013-05-15 发布日期:2013-05-07
通讯作者: 白卫滨 E-mail:baiweibin@163.com
基金资助:
国家自然科学基金项目(31201340)；“十二五”国家科技支撑计划项目(2012BAK01B00)

Effect of 1,3-Dichloro-2-propanol on Cell Growth and Progesterone Biosynthesis of R2C Mouse Leydig Cells

BAI Shun1， SUN Jian-xia2，BAI Wei-bin3,*，ZOU Fei-yan1，ZHANG Qi-hao4，HUANG Ya-dong4

1. Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China；
2. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China；
3. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China；
4. Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China

Received:2012-06-20 Revised:2013-04-11 Online:2013-05-15 Published:2013-05-07
Contact: BAI Wei-bin E-mail:baiweibin@163.com

摘要/Abstract

摘要：

目的：研究1,3-二氯-2-丙醇(1,3-DCP)对体外睾丸间质细胞活性及孕酮合成的影响。方法：分别用0.5、1、2、4、6mmol/L浓度的1,3-DCP作用R2C细胞48h，利用MTT法获得1,3-DCP对R2C细胞的IC25、IC50以及IC75。选取以上3种不同浓度的1,3-DCP作用细胞4h或24h，通过单细胞电泳法(SCGE)检测其作用4h时对DNA损伤的程度；通过放射免疫法(RIA)检测4h或24h处理时对孕酮合成的影响。结果：与对照组相比，1,3-DCP能抑制R2C细胞的增殖，其IC25、IC50、IC75分别为(1.161±0.046)、(1.897±0.018)、(3.100±0.040)mmol/L；作用4h对R2C细胞DNA有损伤作用；各浓度组作用4h或24h后与对照组相比均能降低孕酮的合成。结论：1,3-DCP能抑制R2C的活性，影响细胞孕酮的合成。

关键词: 1,3-二氯-2-丙醇, 睾丸间质细胞, 孕酮, 细胞活性, 机制

Abstract:

Objective: To explore the effect of 1,3-dichloro-2-propanol (1,3-DCP) on cell growth and progesterone biosynthesis
in R2C mouse leydig cells. Methods: R2C cells were exposed to 0.5, 1, 2, 4 mmol/L, and 6 mmol/L 1,3-DCP for 48 h, different
concentrations (IC25, IC50 and IC75) of 1,3-DCP were elected by MTT assay. R2C cells were stimulated by three concentrations
for 4 h or 24 h, respectively. The damage of DNA in R2C cells was investigated by single cell gel electrophoresis (SCGE).
Progesterone level in supernatant of cell culture medium was measured by radioimmunoassay (RIA). Results: 1,3-DCP could
inhibit cell growth, and IC25, IC50 and IC75 values were (1.161 ± 0.046), (1.897 ± 0.018) mmol/L and (3.100 ± 0.040) mmol/L.
In comparison with the control group, the damage of DNA were obviously higher in three concentrations of 1,3-DCP for 4 h,
and the progesterone level were also obviously lower in three concentrations of 1,3-DCP for 4 h or 24 h. Conclusion: 1,3-DCP
can directly affect cell growth and the progesterone synthesis of R2C cells.

Key words: 3-monochloropropane-1,2-diol, leydig cell, progesterone, cell growth, mechanism

中图分类号:

TS201.6

白顺1，孙建霞2，白卫滨3,*，邹飞雁1，张齐好4，黄亚东4. 1,3-二氯-2-丙醇对睾丸间质细胞R2C活性及孕酮合成的影响[J]. 食品科学, 2013, 34(9): 292-295.

BAI Shun1， SUN Jian-xia2，BAI Wei-bin3,*，ZOU Fei-yan1，ZHANG Qi-hao4，HUANG Ya-dong4. Effect of 1,3-Dichloro-2-propanol on Cell Growth and Progesterone Biosynthesis of R2C Mouse Leydig Cells[J]. FOOD SCIENCE, 2013, 34(9): 292-295.

参考文献

王卫华,徐锐锋,刘军等. 食品中氯丙醇测定方法研究进展[J]. 化学分析计量, 2007, 16(3): 74-76.
NYMAN P J, DIACHENKO G W, PERFETTI G A. Survey of chloropropanols in soy sauces and related products[J]. Food Additives and Contaminants, 2003, 20: 909-915.
STEPHEN W C,KWONG K P, JOAN C W, et al. Chloropropanols levels in foodstuffs marketed in Hong Kong[J]. Journal of Food Composition and Analysis, 2008, 21(7): 569-573.
SHIOZAKI T, MIZOBATA Y, SUGIMOTO H, et al. Fulminant hepatitis following exposure to dichlorohydrin report of two cases[J]. Human & Experimental Toxicology, 1994, 13: 267-270.
YANN Grossea, ROBERT Baana, BEATRICE Secretan-Lauby, et al. Carcinogenicity of chemicals in industrial and consumer products, food contaminants and flavourings, and water chlorination byproducts[J]. The Lancet Oncology, 2011, 12(4): 328-329.
LEE J C, SHIN I S, AHN T H, et al. Developmental toxic potential of 1,3-dichloro-2-propanol in Sprague-Dawley rats[J]. Regulatory Toxicology and Pharmacology, 2009, 53: 63-69.
Safety evaluation of certain food additives and contaminants/prepared by the sixty-seventh meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA)[C]. WHO Food Additives Series, 2007, 58: 209-238.
张烨,丁晓雯. 食品中氯丙醇污染及其毒性[J]. 粮食与油脂, 2005, 7: 44-46.
李吉平,高宏伟,刘文森等. 小口径毛细管色谱柱测定酱油中三种氯丙醇含量的研究[J]. 食品科学, 2007, 28(9):473-477.
OMURA M, HIRATA M, ZHAO M, et al. Comparative testicular toxicities of two isomers of dichloropropanol, 2,3-dichloro-l-propanol, and 1,3-dichlo -ro-2-propanol, and their metabolites alpha-chlorohydrin and epichlorohydrin, and the Potent testicular toxicant 1,2-dibromo-3-chloropropane[J]. Bulletin of Environmental Contamination Toxicology, 1995, 55(1): 127.
李宁,刘泽钦,贾旭东等. 氯丙醇对大鼠的毒性研究[J]. 卫生研究, 2003, 32(4):349-352.
KLUWE W M, GUPTA B N , LAMB J C. The comparative effects of 1,2-dibromo-3-chloropropane(DBCP) and its metabolites,3-chloro-1,2-propaneoxide(epichlorohydrin),3-chloro-,1,2-propanediol(alphachlorohydrin), and oxalic acid, on the urogenital system of male rats[J]. Toxicology and Applied Pharmacology, 1983, 70: 67-86.
戴滢. 国际上对（含氯丙醇）盐酸水解蛋白的安全性评价及质量标准[S]. 食品信息指导, 1999, 12: 31-32.
ZHANG Qihao,ZOU Ping,ZHAN Haichao, et al. Dihydrolipoamide dehydrogenase and cAMP are associated with cadmium-mediated Leydig cell damage[J]. Toxicology Letters, 2011, 205: 183-189.
管永波,郝鹏飞,唐春宇等. 氟化钠对小鼠睾丸间质瘤细胞StAR和P450scc mRNA表达的影响[J]. 卫生研究, 2012, 41(1): 105-108.

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1,3-二氯-2-丙醇对睾丸间质细胞R2C活性及孕酮合成的影响

Effect of 1,3-Dichloro-2-propanol on Cell Growth and Progesterone Biosynthesis of R2C Mouse Leydig Cells

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