食品科学

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宏基因组学技术分析传统食醋发酵过程微生物多样性

聂志强,韩 玥,郑 宇,申雁冰,王 敏*   

  1. 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 王 敏
  • 基金资助:

    国家“863”计划项目(2013AA102106);国家自然科学基金项目(31201406);
    教育部“长江学者和创新团队发展计划”项目(IRT1166);高等学校博士学科点专项科研基金项目(20101208120003)

Metagenomic Analysis of Microbial Diversity in the Traditional Vinegar Fermentation Process

NIE Zhi-qiang,HAN Yue,ZHENG Yu,SHEN Yan-bing,WANG Min*   

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology,
    Tianjin University of Science and Technology, Tianjin 300457, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: WANG Min

摘要:

传统食醋具有悠久的历史,生产工艺独特,酿造过程中复杂的微生物群落及其代谢产物赋予了传统食醋独特的风味。采用宏基因组学技术对天津独流老醋醋酸发酵过程中细菌群落组成及其多样性进行分析。结果表明:在醋酸发酵前期细菌具有较高的多样性,主成分分析表明与醋酸发酵过程相关的细菌为乳杆菌属(Lactobacillus)、醋杆菌属(Acetobacter)和念珠藻属(Nostoc)。随着醋酸发酵的进行,醋酸菌的含量呈增加趋势,乳酸菌的丰度降低,在整个醋酸发酵过程中乳酸菌的丰度远远高于其他细菌,说明乳酸菌可能对食醋的风味形成具有重要作用。

关键词: 传统食醋, 宏基因组学, 微生物多样性, 醋酸发酵, 醋酸菌, 乳酸菌

Abstract:

Complex microbial communities and metabolites play important roles in the formation of taste and flavor during
the fermentation process of traditional vinegar. The present study investigated the composition and diversity of bacterial
communities in the acetic acid fermentation process of Tianjin duliu mature vinegar using metagenomic approach. The
results showed that bacteria exhibited greater diversity in the early period than other microorganisms. Principal component
analysis revealed that Lactobacillus, Acetobacter and Nostoc had the main relationship with the acetic acid fermentation
process. The abundance of acetic acid bacteria increased as the fermentation proceeded, while lactic acid bacteria decreased.
The abundance of lactic acid bacteria was far more than that of other microorganisms, indicating that it might have important
functions for the formation of vinegar flavor.

Key words: traditional vinegar, metagenomics, microbial diversity, acetic acid fermentation, acetic acid bacteria, lactic acid bacteria

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