正交试验优化大孔吸附树脂分离纯化杜仲绿原酸

doi:10.7506/spkx1002-6630-201316005

食品科学

正交试验优化大孔吸附树脂分离纯化杜仲绿原酸

熊硕，崔丽，刘仲华，张盛，肖文军

湖南农业大学园艺园林学院，国家植物功能成分工程技术研究中心，湖南长沙 410128

出版日期:2013-08-25 发布日期:2013-09-03

Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin

XIONG Shuo，CUI Li，LIU Zhong-hua，ZHANG Sheng，XIAO Wen-jun

National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients,College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China

Online:2013-08-25 Published:2013-09-03

摘要/Abstract

摘要：

以杜仲叶60%乙醇提取液为原料，研究NKA-2、NKA-9、LS-600、HZ806、HZ906、HPD100、HPD500、S-8八种大孔吸附树脂对绿原酸的静态吸附与解吸效果，优化最优树脂分离纯化杜仲叶绿原酸的工艺技术参数。结果表明：NKA-2树脂对绿原酸具有较好的吸附与解吸性能，在pH3的条件下静态吸附量为86mg/g，解吸率达93.23%；当主要考虑绿原酸得率时，最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为2BV，在该工艺参数条件下，绿原酸得率为83.14%，纯度为28.91%；当主要考虑绿原酸纯度时，最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为1.5BV，在该工艺参数条件下，绿原酸得率为79.62%，纯度为39.38%。

关键词: 杜仲叶, 绿原酸, 大孔吸附树脂, 分离纯化

Abstract:

Different types of macroporous adsorption resin, including NKA-2, NKA-9, LS-600, HZ806, HZ906,
HPD100, HPD500 and S-8 were compared for their static adsorption and desorption capacities for chlorogenic acid in
60% ethanol extract of Eucommia ulmoidues leaves. NKA-2 was found to have a stronger ability to adsorb and desorb
chlorogenic acid and the optimal operating parameters for purifying chlorogenic acid from the ethanol extract using
NKA-2 were determined. The static adsorption quantity of chlorogenic acid on NKA-2 and desorption percentage were
86 mg/g and 93.23%, respectively. The optimal process conditions that provided maximum chlorogenic acid recovery
(83.14%; purity 28.91%) were determined as 4 mg/mL, 4 BV/h, 5 BV, 80%, 3 BV/h and 2 BV for sample concentration,
loading flow rate, loading volume, desorbent concentration, desorption flow rate, and desorbent volume, respectively,
and whereas those for maximum chlorogenic acid purity (39.38%; recovery 79.62% ) were 4 mg/mL, 4 BV/h, 5 BV,
80%, 3 BV/h and 1.5 BV, respectively.

Key words: Eucommia ulmoidues leaves, chlorogenic acid, macroporous resin, separation and purification

熊硕，崔丽，刘仲华，张盛，肖文军. 正交试验优化大孔吸附树脂分离纯化杜仲绿原酸[J]. 食品科学, doi: 10.7506/spkx1002-6630-201316005.

XIONG Shuo，CUI Li，LIU Zhong-hua，ZHANG Sheng，XIAO Wen-jun. Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316005.

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正交试验优化大孔吸附树脂分离纯化杜仲绿原酸

Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin

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