食品科学

• 分析检测 • 上一篇    下一篇

基于石墨烯-壳聚糖修饰电极的免疫传感器检测三聚氰胺

邵科峰,陈昌云,颜 妍,吴 珺,赵 波   

  1. 1.南京师范大学化学与材料科学学院,江苏 南京 210023;
    2.南京晓庄学院生物化工与环境工程学院,江苏 南京 211171
  • 出版日期:2013-08-25 发布日期:2013-09-03

Electrochemical Immunosensor for Melamine Determination Based on Graphene-Chitosan Composite Film Modified Electrode

SHAO Ke-feng,CHEN Chang-yun,YAN Yan,WU Jun,ZHAO Bo   

  1. 1. School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China;
    2. School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

构建一种基于石墨烯-壳聚糖修饰电极的检测三聚氰胺的电化学免疫传感器。利用三聚氰胺抗体和三聚氰胺之间特定反应的竞争模式,以K3Fe(CN)6为探针,通过循环伏安法和差分脉冲伏安法监测免疫反应,对溶液中的三聚氰胺进行检测。检测线性范围宽(5~1500ng/mL),检出限可低至0.2ng/mL。同时,对牛奶实际样品进行测定,回收率为104.0%~106.2%。

关键词: 石墨烯, 壳聚糖, 三聚氰胺, 电化学免疫传感器

Abstract:

An electrochemical immunosensor for the determination of melamine based on graphene-chitosan modified
electrode was constructed. Indirect competitive electrochemical immunoassay was performed to determine melamine in
aqueous solutions. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were carried out to monitor the
fabrication steps of immunoreaction system in solutions containing K3Fe(CN)6 as redox probe. The immunosensor demonstrated
excellent melamine detection features with a broad linear range of 5 to 1500 ng/mL and a detection limit of 0.2 ng/mL. A good
recovery of melamine in the range of 104%–106.2% was obtained in spiked milk samples.

Key words: graphene, chitosan, melamine, electrochemical immunosensor