食品科学

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共振散射光猝灭法测定小扁豆和贼小豆中微量硒

高向阳,吴云静,王莹莹   

  1. 1. 郑州科技学院食品科学与工程学院,河南 郑州 450064;2.河南农业大学食品科学技术学院,河南 郑州 450002
  • 出版日期:2013-08-25 发布日期:2013-09-03

Determination of Microamounts of Selenium in Seeds of Lens culinaris and Vigna minima by Resonance Scattering Light Quenching Method

GAO Xiang-yang,WU Yun-jing,WANG Ying-ying   

  1. 1. School of Food Science and Engineering, University for Science and Technology Zhengzhou, Zhengzhou 450064, China;
    2. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

建立一种共振散射光猝灭法测定粮食中微量硒的新方法。以小扁豆和贼小豆为样品,用pH3.500的B-R缓冲溶液为介质,硒(Ⅳ)与一定量的I-反应生成等物质的量I3-,进一步与阳离子染料罗丹明6G反应生成离子缔合物,引起592.2nm共振散射光的线性猝灭。结果表明:在最佳测定条件下,测定体系共振散射光的相对猝灭强度ΔI与硒在0~60μg/L质量浓度范围内有良好线性关系,方法的检出限为1.710μg/L,小扁豆和贼小豆硒含量测定的相对标准偏差(RSD)分别为3.9%、1.9%(n=7),回收率分别为95.56%、96.80%,测定结果令人满意。

关键词: 小扁豆, 贼小豆, 硒, 共振散射光猝灭法

Abstract:

A new method to determine microamounts of selenium in the seeds of Lens culinaris and Vigna minima was
established using resonance scattering light quenching. In pH 3.500 B-R buffer solution, selenium (Ⅳ) reacted with a certain
amount of I- to produce an equal molar amount of I3-, which further reacted with rhodamine 6G to form an ion association
complex causing linear quenching of resonance scattering light at 592.2 nm. The results showed good linear relationship
between resonance scattering light relative quenching intensity ΔI and Se(Ⅳ) concentration in the range of 0–60 μg/L under
optimized conditions. The detection limit of the method was 1.710 μg/L. The precision (RSD) for 7 replicate measurements
of selenium in the seeds of Lens culinaris and Vigna minima was 3.9% and 1.9%, respectively, and the average spiked
recovery was 95.56% and 96.80%, respectively. These results were satisfactory.

Key words: lentils (Lens culinaris), Vigna minima, selenium, resonance scattering light quenching method